| The Ton system, an ABC transporter, and a universally conserved GTPase are involved in iron utilization by Brucella melitensis 16M. | |
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MedLine Citation:
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PMID: 15385478 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Brucella spp. are gram-negative intracellular facultative pathogens that are known to produce 2,3-dihydroxybenzoic acid (DHBA), a catechol siderophore that is essential for full virulence in the natural host. The mechanism of DHBA entry into Brucella and other gram-negative bacteria is poorly understood. Using mini-Tn5Kmcat mutagenesis, we created a transposon library of Brucella melitensis 16M and isolated 32 mutants with a defect in iron acquisition or assimilation. Three of these transposon mutants are deficient in utilization of DHBA. Analysis of these three mutants indicated that the ExbB, DstC, and DugA proteins are required for optimal assimilation of DHBA and/or citrate. ExbB is part of the Ton complex, and DstC is a permease homologue of an iron(III) ABC transporter; in gram-negative bacteria these two complexes are involved in the uptake of iron through the outer and inner membranes, respectively. DugA is a new partner in iron utilization that exhibits homology with the bacterial conserved GTPase YchF. Based on this homology, DugA could have a putative regulatory function in iron assimilation in Brucella. None of the three mutants was attenuated in cellular models or in the mouse model of infection, which is consistent with the previous suggestion that DHBA utilization is not required in these models. |
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Authors:
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Isabelle Danese; Valerie Haine; Rose-May Delrue; Anne Tibor; Pascal Lestrate; Olivier Stevaux; Pascal Mertens; Jean-Yves Paquet; Jacques Godfroid; Xavier De Bolle; Jean-Jacques Letesson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Infection and immunity Volume: 72 ISSN: 0019-9567 ISO Abbreviation: Infect. Immun. Publication Date: 2004 Oct |
Date Detail:
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Created Date: 2004-09-23 Completed Date: 2004-10-25 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: United States |
Other Details:
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Languages: eng Pagination: 5783-90 Citation Subset: IM |
Affiliation:
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Unité de Recherche en Biologie Moléculaire, University of Namur, Belgium. Isabelle.Danese@fundp.ac.be |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AF325201; AF358662; AY028973 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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ATP-Binding Cassette Transporters
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genetics,
metabolism* Amino Acid Sequence Animals Bacterial Proteins / chemistry, genetics, metabolism* Brucella melitensis / classification*, drug effects, genetics, metabolism* Brucellosis / microbiology Catechols / metabolism, pharmacology Cattle Colony Count, Microbial GTP Phosphohydrolases / chemistry, genetics, metabolism* Genes, Bacterial / genetics Hela Cells Humans Iron / antagonists & inhibitors, metabolism* Iron Chelating Agents / pharmacology Macrophages / microbiology Mice Mice, Inbred BALB C Molecular Sequence Data Mutation / genetics Siderophores / metabolism, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/2,3-dihydroxy benzoic acid; 0/ATP-Binding Cassette Transporters; 0/Bacterial Proteins; 0/Catechols; 0/Iron Chelating Agents; 0/Siderophores; 7439-89-6/Iron; EC 3.6.1.-/GTP Phosphohydrolases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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