Document Detail

Tomosyn negatively regulates both synaptic transmitter and neuropeptide release at the C. elegans neuromuscular junction.
MedLine Citation:
PMID:  17627987     Owner:  NLM     Status:  MEDLINE    
The SNARE proteins, syntaxin, SNAP-25 and synaptobrevin form a tertiary complex essential for vesicle fusion. Proteins that influence SNARE complex assembly are therefore likely to be important regulators of fusion events. In this study we have focused on tomosyn, a highly conserved, neuronally enriched, syntaxin-binding protein that has been implicated in the regulation of vesicle exocytosis. To directly test the role of tomosyn in neurosecretion we analysed loss-of-function mutants in the single Caenorhabditis elegans tomosyn gene, tom-1. These mutants exhibit enhanced synaptic transmission based on electrophysiological analysis of neuromuscular junction activity. This phenotype is the result of increased synaptic vesicle priming. In addition, we present evidence that tom-1 mutants also exhibit enhanced peptide release from dense core vesicles. These results indicate that tomosyn negatively regulates secretion for both vesicle types, possibly through a common mechanism, interfering with SNARE complex formation, thereby inhibiting vesicle fusion.
Elena O Gracheva; Anna O Burdina; Denis Touroutine; Martine Berthelot-Grosjean; Hetal Parekh; Janet E Richmond
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2007-07-12
Journal Detail:
Title:  The Journal of physiology     Volume:  585     ISSN:  0022-3751     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-12-17     Completed Date:  2008-02-25     Revised Date:  2014-09-19    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  705-9     Citation Subset:  IM    
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MeSH Terms
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / genetics,  physiology*
Neuromuscular Junction / metabolism,  physiology*
Neurotransmitter Agents / metabolism*
Synapses / metabolism,  physiology*
Grant Support
R01 MH 073156/MH/NIMH NIH HHS; R01 MH073156/MH/NIMH NIH HHS; R01 MH073156-01A2/MH/NIMH NIH HHS; R01 MH073156-02/MH/NIMH NIH HHS
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/Neurotransmitter Agents; 0/tom-1 protein, C elegans

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