| Tomitella biformata gen. nov., sp. nov., a new member of the suborder Corynebacterineae isolated from a permafrost ice wedge. | |
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MedLine Citation:
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PMID: 20081023 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Gram-reaction-positive, aerobic, non-spore-forming, irregular rod-shaped bacteria, designated AHU1821(T) and AHU1820, were isolated from an ice wedge in the Fox permafrost tunnel, Alaska. The strains were psychrophilic, growing at -5 to 27 °C. Phylogenetic analysis of the 16S rRNA and gyrB gene sequences indicated that the ice-wedge isolates formed a clade distinct from other mycolic-acid-containing bacteria within the suborder Corynebacterineae. The cell wall of strains AHU1821(T) and AHU1820 contained meso-diaminopimelic acid, arabinose and galactose, indicating chemotype IV. The muramic acids in the peptidoglycan were glycolated. The predominant menaquinone was MK-9(H(2)). The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and an unidentified glycolipid. The major fatty acids were hexadecenoic acid (C(16 : 1)), hexadecanoic acid (C(16 : 0)), octadecenoic acid (C(18 : 1)) and tetradecanoic acid (C(14 : 0)). Tuberculostearic acid was present in relatively small amounts (1 %). Strains AHU1821(T) and AHU1820 contained mycolic acids with 42-52 carbons. The DNA G+C content of the two strains was 69.3-71.6 mol% (T(m)). 16S rRNA, rpoB and recA gene sequences were identical between strains AHU1821(T) and AHU1820 and those of the gyrB gene showed 99.9 % similarity. Based on phylogenetic and phenotypic evidence, strains AHU1821(T) and AHU1820 represent a single novel species of a novel genus, for which the name Tomitella biformata gen. nov., sp. nov. is proposed. The type strain of Tomitella biformata is AHU1821(T) (=DSM 45403(T) =NBRC 106253(T)). |
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Authors:
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Taiki Katayama; Tomoko Kato; Michiko Tanaka; Thomas A Douglas; Anatoli Brouchkov; Ayumi Abe; Teruo Sone; Masami Fukuda; Kozo Asano |
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Publication Detail:
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Type: Journal Article Date: 2010-01-15 |
Journal Detail:
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Title: International journal of systematic and evolutionary microbiology Volume: 60 ISSN: 1466-5034 ISO Abbreviation: Int. J. Syst. Evol. Microbiol. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100899600 Medline TA: Int J Syst Evol Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 2803-7 Citation Subset: IM |
Affiliation:
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Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University, N9 W9, Kita-ku, Hokkaido 060-8589, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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