Document Detail


Tomato profilin Lyc e 1: IgE cross-reactivity and allergenic potency.
MedLine Citation:
PMID:  15080834     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: To date, very little data are available about the nature of tomato allergens. Immunoglobulin E (IgE) cross-reactive profilins have been suggested to account for allergic symptoms in patients suffering from tomato allergy. METHODS: The cDNA of tomato profilin was amplified by reversely transcribed polymerase chain reaction (RT-PCR) from total RNA extracted from ripe tomato fruit. The gene was cloned into the pET101D expression plasmid and the protein was produced in Escherichia coli BL21. Purification was performed via poly-l-proline (PLP) affinity chromatography. IgE reactivity of recombinant tomato profilin was investigated by immunoblot and enzyme-linked immunosorbent assay. IgE-inhibition studies were performed to analyse cross-reactivity with other profilins. To determine the allergenic activity of the recombinant protein, basophil histamine release assays using sera of patients with adverse reactions to tomato were performed. RESULTS: Profilin was identified as a new minor allergen in tomato fruits. The recombinant tomato profilin comprises 131 amino acids and high sequence identity to other allergenic food and pollen profilins. It was shown to be IgE-reactive with a prevalence of 22% (11/50) in tomato-allergic patients. In patients with tomato allergy and multiple sensitization to other foods and birch pollen, IgE directed against tomato profilin showed a strong cross-reactivity with profilins from plant food sources and birch pollen. The tomato profilin was able to induce mediator release from human basophils. CONCLUSION: The tomato profilin is a minor allergen in tomato fruit. Thus, it shows biological activity, as confirmed by in vitro histamine release assays with human basophils and thereby has the potential to account for clinical symptoms in tomato-allergic patients.
Authors:
S Westphal; W Kempf; K Foetisch; M Retzek; S Vieths; S Scheurer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Allergy     Volume:  59     ISSN:  0105-4538     ISO Abbreviation:  Allergy     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-04-14     Completed Date:  2004-08-09     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  7804028     Medline TA:  Allergy     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  526-32     Citation Subset:  IM    
Affiliation:
Department of Allergology, Paul-Ehrlich-Institut, Langen, Germany.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AJ417552;  AJ417553
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MeSH Terms
Descriptor/Qualifier:
Adult
Allergens / genetics,  immunology*,  metabolism
Amino Acid Sequence
Basophils / metabolism
Betula / immunology
Cloning, Molecular
Contractile Proteins / genetics,  immunology*,  metabolism
Cross Reactions
DNA, Complementary
Escherichia coli / metabolism
Female
Food Hypersensitivity / etiology*,  immunology*
Histamine Release
Humans
Immunization
Immunoglobulin E / immunology*
Lycopersicon esculentum / adverse effects*,  immunology*
Male
Microfilament Proteins / genetics,  immunology*,  metabolism
Middle Aged
Molecular Sequence Data
Pollen / immunology
Profilins
Recombinant Proteins / immunology
Chemical
Reg. No./Substance:
0/Allergens; 0/Contractile Proteins; 0/DNA, Complementary; 0/Lyc e 1 allergen, Lycopersicon esculentum; 0/Microfilament Proteins; 0/Profilins; 0/Recombinant Proteins; 37341-29-0/Immunoglobulin E

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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