Document Detail


Tomato phenylacetaldehyde reductases catalyze the last step in the synthesis of the aroma volatile 2-phenylethanol.
MedLine Citation:
PMID:  17644147     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The volatile compounds, 2-phenylacetaldehyde and 2-phenylethanol, are important for the aroma and flavor of many foods, such as ripe tomato fruits, and are also major constituents of scent of many flowers, most notably roses. While much work has gone into elucidating the pathway for 2-phenylethanol synthesis in bacteria and yeast, the pathways for synthesis in plants are not well characterized. We have identified two tomato enzymes (LePAR1 and LePAR2) that catalyze the conversion of 2-phenylacetaldehyde to 2-phenylethanol: LePAR1, a member of the large and diverse short-chain dehydrogenase/reductase family, strongly prefers 2-phenylacetaldehyde to its shorter and longer homologues (benzaldehyde and cinnamaldehyde, respectively) and does not catalyze the reverse reaction at a measurable rate; LePAR2, however, has similar affinity for 2-phenylacetaldehyde, benzaldehyde and cinnamaldehyde. To confirm the activity of these enzymes in vivo, LePAR1 and LePAR2 cDNAs were individually expressed constitutively in petunia. While wild type petunia flowers emit relatively high levels of 2-phenylacetaldehyde and lower levels of 2-phenylethanol, flowers from the transgenic plants expressing LePAR1 or LePAR2 had significantly higher levels of 2-phenylethanol and lower levels of 2-phenylacetaldehyde. The in vivo alteration of volatile emissions is an important step toward altering aroma volatiles in plants.
Authors:
Denise M Tieman; Holly M Loucas; Joo Young Kim; David G Clark; Harry J Klee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-07-17
Journal Detail:
Title:  Phytochemistry     Volume:  68     ISSN:  0031-9422     ISO Abbreviation:  Phytochemistry     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-29     Completed Date:  2008-01-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0151434     Medline TA:  Phytochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2660-9     Citation Subset:  IM    
Affiliation:
Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, United States.
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MeSH Terms
Descriptor/Qualifier:
Alcohol Oxidoreductases / chemistry,  isolation & purification,  metabolism*
Catalysis
DNA, Complementary / biosynthesis
Lycopersicon esculentum / enzymology*,  genetics
Molecular Structure
Oxidation-Reduction
Petunia / genetics
Phenylethyl Alcohol / metabolism*
Plants, Genetically Modified
Volatilization
Chemical
Reg. No./Substance:
0/DNA, Complementary; 60-12-8/Phenylethyl Alcohol; EC 1.1.-/Alcohol Oxidoreductases; EC 1.1.1-/phenylacetaldehyde reductase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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