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Toll-like receptor 7 agonist, Imiquimod, inhibits oral squamous carcinoma cells through apoptosis and necrosis.
MedLine Citation:
PMID:  22577802     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
J Oral Pathol Med (2012) Background:  Toll-like receptor (TLR) agonists have anticancer effect by inducing apoptosis or activating immune cells. In this study, we investigated whether imiquimod, TLR7 agonist, inhibits the proliferation of oral cancer cells. Methods:  Toll-like receptor 7 expression and IL-6/8 production by imiquimod were examined using RT-PCR and Enzyme-linked immunosorbent assay, respectively. Cell viability was examined by MTT assay. To examine apoptotic cell death, Annexin V/PI staining for flow cytometry and Western blot analysis were performed. Necrotic cell death was determined by leakage of lactate dehydrogenase (LDH), HMGB1, and PI staining in imiquimod-treated oral squamous cell carcinoma (OSCC) cells. Results:  Toll-like receptor7 mRNA was expressed in OSCC cells. Imiquimod induced IL-6 and IL-8 production in OSCC cells, suggesting the functional expression of TLR7. Imiquimod inhibited cells proliferation in a dose-dependent manner. The ratio of annexin V-positive cells and cleaved caspase-3/7 was increased by imiquimod treatment in OSCC cells, suggesting that imiquimod-induced cell death in OSCC cells may be owing to apoptosis. In addition, LDH secretion and PI staining were detected in OSCC cells treated with imiquimod, showing that imiquimod also induced necrotic cell death in the OSCC cells. Conclusions:  Imiquimod inhibited effectively the growth of OSCC cells by inducing apoptosis and necrosis.
Authors:
Mee-Young Ahn; Seong-Min Kwon; Hak Hyun Cheong; Jong-Hwan Park; Jun Lee; Seung-Ki Min; Sang-Gun Ahn; Jung-Hoon Yoon
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-12
Journal Detail:
Title:  Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology     Volume:  -     ISSN:  1600-0714     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8911934     Medline TA:  J Oral Pathol Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 John Wiley & Sons A/S.
Affiliation:
Department of Pathology and Research Center for Oral Disease Regulation of the Aged, School of Dentistry, Chosun University, Gwangju, Korea Department of Biochemistry, College of Medicine, Konyang University, Daejeon, Korea Department of Oral and Maxillofacial Surgery, College of Dentistry, Daejeon Dental Hospital, Wonkwang Bone Regeneration Research Institute, Wonkwang University, Daejeon, Korea Department of Oral and Maxillofacial Pathology, College of Dentistry, Daejeon Dental Hospital, Wonkwang Bone Regeneration Research Institute, Wonkwang University, Daejeon, Korea.
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