Document Detail


Toll-like receptor 4 modulates cell migration and cortical neurogenesis after focal cerebral ischemia.
MedLine Citation:
PMID:  25063846     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Toll-like receptor 4 (TLR4) mediates brain damage after stroke. Now our objective is to determine TLR4 involvement in stroke-induced neurogenesis. Stroke was induced by permanent middle cerebral artery occlusion in wild-type and TLR4-deficient mice. Stereological and densitometric analysis of immunofluorescence-labeled brain sections and FACS analysis of cell suspensions were performed. Our results show that subventricular zone (SVZ) cell proliferation after stroke depends on infarct size. Second, when comparing brains with similar lesions, TLR4 attenuated SVZ proliferation, as shown by a decrease in prominin-1(+)/EGFR(+)/nestin(-) cells (type-C cells) at 1-2 d, and in BrdU(+) cells at 7 d, in TLR4(+/+) vs. TLR4(-/-) mice. Interestingly, 7 d after the infarct, neuroblasts in TLR4(+/+) mice migrated farther distances, reaching areas closer to the lesion than those in TLR4-deficient mice. However, at 14 d, TLR4-deficient mice presented a higher number of neuroblasts in all migratory zones than the TLR4(+/+) counterparts, which suggests that TLR4 deficiency delays neuroblast migration. Consistently, TLR4(+/+) mice showed an increased number of interneurons (NeuN(+)/BrdU(+)/GAD67(+) cells) in peri-infarct cortex 14-28 d after stroke. Our data indicate that, despite a negative effect on SVZ cell proliferation, TLR4 plays an important role in stroke-induced neurogenesis by promoting neuroblasts migration and increasing the number of new cortical neurons after stroke.-Moraga, A., Pradillo, J. M., Cuartero, M. I., Hernández-Jiménez, M., Oses, M., Moro, M. A., Lizasoain, I. Toll-like receptor 4 modulates cell migration and cortical neurogenesis after focal cerebral ischemia.
Authors:
Ana Moraga; Jesús M Pradillo; María I Cuartero; Macarena Hernández-Jiménez; Marta Oses; María A Moro; Ignacio Lizasoain
Related Documents :
24885036 - Lack of synergistic effect of resveratrol and sigma-1 receptor agonist (pre-084) in sod...
24763046 - Adiponectin deficiency exacerbates age-related hearing impairment.
23079716 - Overexpression of human spata17 protein induces germ cell apoptosis in transgenic male ...
24014876 - Ifit2 is an effector protein of type i ifn-mediated amplification of lipopolysaccharide...
8020556 - A genetic association between systemic lupus erythematosus and tumor necrosis factor al...
9884336 - Monocyte chemoattractant protein-1 promotes macrophage-mediated tubular injury, but not...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-7-25
Journal Detail:
Title:  FASEB journal : official publication of the Federation of American Societies for Experimental Biology     Volume:  -     ISSN:  1530-6860     ISO Abbreviation:  FASEB J.     Publication Date:  2014 Jul 
Date Detail:
Created Date:  2014-7-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8804484     Medline TA:  FASEB J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© FASEB.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Sensitivity and specificity of the empirical lymphocyte genome sensitivity (LGS) assay: implications...
Next Document:  The light-dark cycle controls peripheral rhythmicity in mice with a genetically ablated suprachiasma...