Document Detail

Toll-like receptor-4 and cytokine cascade in stroke after exercise.
MedLine Citation:
PMID:  19726014     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: As part of the innate immune system, activation of Toll-like receptor-4 by inflammatory mediators causes the release of inflammatory cytokines, leading to cellular damage. Exercise for 3 weeks has been shown to provide neuroprotection against ischemia/reperfusion insults by decreasing inflammation. This study explores the expression of Toll-like receptor-4 in brain parenchyma during an ischemic stroke after exercise, in association with brain injury. METHODS: Adult male Sprague-Dawley rats were studied in four groups (control, exercise, stroke and stroke after exercise). Exercise consisted of 30 minutes of treadmill running daily for 3 weeks. Using an intraluminal filament, a stroke was induced by 2 hour middle cerebral artery occlusion. Nissl staining was used to determine brain infarct volume. Brain Toll-like receptor-4 messenger RNA expression and protein levels were determined by real time reverse transcriptase polymerase chain reaction and Western blot, respectively. RESULTS: Exercise significantly (p<0.05) decreased Toll-like receptor-4 messenger RNA and protein expression, in association with significantly (p<0.05) reduced brain infarct volume. The stroke group had significantly (p<0.05) increased levels of Toll-like receptor-4 expression compared to the control, whereas the exercise/stroke group was significantly (p<0.05) attenuated. CONCLUSION: Exercise reduced Toll-like receptor-4 expression within brain tissue in response to ischemia/reperfusion insult. This is the first paper, to our knowledge, indicating the correlation between exercise, Toll-like receptor-4 expression and reduced brain infarct volume.
Nathan Zwagerman; Chuck Plumlee; Murali Guthikonda; Yuchuan Ding
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2009-09-01
Journal Detail:
Title:  Neurological research     Volume:  32     ISSN:  1743-1328     ISO Abbreviation:  Neurol. Res.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-17     Completed Date:  2010-09-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7905298     Medline TA:  Neurol Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  123-6     Citation Subset:  IM    
Department of Neurosurgery, Wayne State University School of Medicine, Detroit, MI, USA.
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MeSH Terms
Brain Infarction / metabolism,  physiopathology,  prevention & control
Cytokines / biosynthesis*,  secretion
Exercise Test / methods
Infarction, Middle Cerebral Artery / metabolism,  physiopathology
Ischemic Preconditioning / methods
Physical Conditioning, Animal* / methods
Random Allocation
Rats, Sprague-Dawley
Stroke / metabolism*,  physiopathology
Toll-Like Receptor 4 / biosynthesis*
Reg. No./Substance:
0/Cytokines; 0/Tlr4 protein, rat; 0/Toll-Like Receptor 4

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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