| Toll-like receptor-4 and cytokine cascade in stroke after exercise. | |
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MedLine Citation:
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PMID: 19726014 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: As part of the innate immune system, activation of Toll-like receptor-4 by inflammatory mediators causes the release of inflammatory cytokines, leading to cellular damage. Exercise for 3 weeks has been shown to provide neuroprotection against ischemia/reperfusion insults by decreasing inflammation. This study explores the expression of Toll-like receptor-4 in brain parenchyma during an ischemic stroke after exercise, in association with brain injury. METHODS: Adult male Sprague-Dawley rats were studied in four groups (control, exercise, stroke and stroke after exercise). Exercise consisted of 30 minutes of treadmill running daily for 3 weeks. Using an intraluminal filament, a stroke was induced by 2 hour middle cerebral artery occlusion. Nissl staining was used to determine brain infarct volume. Brain Toll-like receptor-4 messenger RNA expression and protein levels were determined by real time reverse transcriptase polymerase chain reaction and Western blot, respectively. RESULTS: Exercise significantly (p<0.05) decreased Toll-like receptor-4 messenger RNA and protein expression, in association with significantly (p<0.05) reduced brain infarct volume. The stroke group had significantly (p<0.05) increased levels of Toll-like receptor-4 expression compared to the control, whereas the exercise/stroke group was significantly (p<0.05) attenuated. CONCLUSION: Exercise reduced Toll-like receptor-4 expression within brain tissue in response to ischemia/reperfusion insult. This is the first paper, to our knowledge, indicating the correlation between exercise, Toll-like receptor-4 expression and reduced brain infarct volume. |
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Authors:
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Nathan Zwagerman; Chuck Plumlee; Murali Guthikonda; Yuchuan Ding |
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Publication Detail:
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Type: Comparative Study; Journal Article Date: 2009-09-01 |
Journal Detail:
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Title: Neurological research Volume: 32 ISSN: 1743-1328 ISO Abbreviation: Neurol. Res. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-17 Completed Date: 2010-09-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7905298 Medline TA: Neurol Res Country: England |
Other Details:
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Languages: eng Pagination: 123-6 Citation Subset: IM |
Affiliation:
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Department of Neurosurgery, Wayne State University School of Medicine, Detroit, MI, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain Infarction / metabolism, physiopathology, prevention & control Cytokines / biosynthesis*, secretion Exercise Test / methods Infarction, Middle Cerebral Artery / metabolism, physiopathology Ischemic Preconditioning / methods Male Physical Conditioning, Animal* / methods Random Allocation Rats Rats, Sprague-Dawley Stroke / metabolism*, physiopathology Toll-Like Receptor 4 / biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/Tlr4 protein, rat; 0/Toll-Like Receptor 4 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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