Document Detail


Toll-like receptor 2 and facial motoneuron survival after facial nerve axotomy.
MedLine Citation:
PMID:  20056129     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have previously demonstrated that CD4(+) Th2 lymphocytes are required to rescue facial motoneuron (FMN) survival after facial nerve axotomy through interaction with peripheral antigen presenting cells, as well as CNS resident microglia. Furthermore, the innate immune molecule, toll-like receptor 2 (TLR2), has been implicated in the development of Th2-type immune responses and can be activated by intracellular components released by dead or dying cells. The role of TLR2 in the FMN response to axotomy was explored in this study, using a model of facial nerve axotomy at the stylomastoid foramen in the mouse, in which blood-brain-barrier (BBB) permeability does not occur. After facial nerve axotomy, TLR2 mRNA was significantly upregulated in the facial motor nucleus and co-immunofluorescence localized TLR2 to CD68(+) microglia, but not GFAP(+) astrocytes. Using TLR2-deficient (TLR2(-/-)) mice, it was determined that TLR2 does not affect FMN survival levels after axotomy. These data contribute to understanding the role of innate immunity after FMN death and may be relevant to motoneuron diseases, such as amyotrophic lateral sclerosis (ALS).
Authors:
Derek A Wainwright; Junping Xin; Nichole A Mesnard; Virginia M Sanders; Kathryn J Jones
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-01-05
Journal Detail:
Title:  Neuroscience letters     Volume:  471     ISSN:  1872-7972     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-15     Completed Date:  2010-04-05     Revised Date:  2014-04-08    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  10-4     Citation Subset:  IM    
Copyright Information:
2010 Elsevier Ireland Ltd. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD / metabolism
Antigens, Differentiation, Myelomonocytic / metabolism
Astrocytes / immunology,  metabolism
Axotomy
Cell Survival
Facial Nerve / pathology*
Female
Fluorescent Antibody Technique
Immunity, Innate
Mice
Mice, Inbred C57BL
Mice, Knockout
Microglia / immunology,  metabolism
Motor Neurons / pathology*
Nerve Tissue Proteins / metabolism
Oligonucleotide Array Sequence Analysis
RNA, Messenger / biosynthesis
Th2 Cells / immunology
Toll-Like Receptor 2 / biosynthesis,  genetics,  immunology*
Toll-Like Receptor 4 / biosynthesis,  genetics
Up-Regulation
Grant Support
ID/Acronym/Agency:
NS40433/NS/NINDS NIH HHS; R01 NS040433-09/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, Differentiation, Myelomonocytic; 0/CD68 antigen, human; 0/Nerve Tissue Proteins; 0/RNA, Messenger; 0/Tlr2 protein, mouse; 0/Tlr4 protein, mouse; 0/Toll-Like Receptor 2; 0/Toll-Like Receptor 4; 0/glial fibrillary astrocytic protein, mouse
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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