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Toll-like Receptor 2 Knockout Mice Showed Increased Periapical Lesion Size and Osteoclast Number.
MedLine Citation:
PMID:  22595116     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
INTRODUCTION: The aim of this study was to characterize the formation and progression of experimentally induced periapical lesions in TLR2 knockout (TLR2 KO) mice.
METHODS: Periapical lesions were induced in molars of 28 wild type (WT) and 27 TLR2 KO mice. After 7, 21, and 42 days, the animals were euthanized, and the mandibles were subjected to histotechnical processing. Hematoxylin-eosin-stained sections were examined under conventional light microscopy for the description of pulpal, apical, and periapical features and under fluorescence microscopy for the determination of the periapical lesion size. The subsequent sections were evaluated by tartrate resistant acid phosphatase histoenzymology (osteoclasts), Brown and Brenn staining (bacteria), and immunohistochemistry (RANK, RANKL, and OPG). Data were analyzed by the Mann-Whitney U and Kruskal-Wallis tests (α = 0.05).
RESULTS: The WT group showed significant differences (P < .05) in the periapical lesion size and the osteoclast number between 7 and 42 days and between 21 and 42 days. In the TLR2 KO group, significant differences (P < .05) in the periapical lesion size and the osteoclast number were found between 7 days and the other periods. There was a significant difference (P < .05) between the 2 types of animal regarding the periapical lesion size, which was larger in the TLR2 KO animals. No significant differences (P > .05) were found between WT and TLR2 KO mice related to the pulpal, apical, and periapical features; bacteria localization; and immunohistochemical results (except for RANK expression).
CONCLUSIONS: TLR2 KO animals developed larger periapical lesions with a greater number of osteoclasts, indicating the important role of this receptor in the host's immune and inflammatory response to root canal and periradicular infection.
Authors:
Raquel Assed Bezerra da Silva; Paula Dariana Fernandes Ferreira; Andiara De Rossi; Paulo Nelson-Filho; Lea Assed Bezerra Silva
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Publication Detail:
Type:  Journal Article     Date:  2012-04-26
Journal Detail:
Title:  Journal of endodontics     Volume:  38     ISSN:  1878-3554     ISO Abbreviation:  J Endod     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-05-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7511484     Medline TA:  J Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  803-13     Citation Subset:  D    
Copyright Information:
Copyright © 2012 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
Affiliation:
Department of Pediatric Clinics, Preventive and Community Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
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