| Tolerance induction by intrathymic inoculation prevents chronic renal allograft rejection. | |
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MedLine Citation:
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PMID: 9458029 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: These experiments investigated the ability of the donor-specific unresponsiveness created by the intrathymic inoculation of donor alloantigen to effectively prevent chronic rejection in an established rat model of chronic renal allograft rejection. METHODS: Three study groups were examined: (1) Allograft controls--F-344 rats received a Lewis renal allograft plus 10 days of low-dose cyclosporine (CsA); (2) isograft controls--F-344 rats received an F-344 renal isograft and low-dose CsA; (3) experimental group--F-344 rats received a T-cell depleted syngeneic bone marrow transplant and intrathymic injection of Lewis bone marrow. Twenty-one days after bone marrow transplant, these animals received a Lewis renal allograft. RESULTS: Allograft controls demonstrated severe parenchymal fibrosis; isograft controls and intrathymic (IT) animals failed to develop this lesion. Immunohistochemical analysis revealed increased CD4+ T cells infiltrating the cortex of the allograft controls. Cytokine interferon-gamma and interleukin-2 transcripts were strongly positive in allograft controls and were absent from isograft controls and IT allografts as determined by reverse transcriptase-polymerase chain reaction. Analysis of tolerant grafts by flow microfluorimetry and genomic DNA amplification could not detect chimerism to a level of < 0.1%. CONCLUSION: IT inoculation of donor alloantigen can confer long-term unresponsiveness and prevent the development of the characteristic lesions of chronic rejection. |
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Authors:
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D Blom; N Morrissey; C Mesonero; X J Zuo; S Jordan; T Fisher; O Bronsther; M S Orloff |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Transplantation Volume: 65 ISSN: 0041-1337 ISO Abbreviation: Transplantation Publication Date: 1998 Jan |
Date Detail:
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Created Date: 1998-02-11 Completed Date: 1998-02-11 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0132144 Medline TA: Transplantation Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 272-5 Citation Subset: IM |
Affiliation:
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Department of Surgery, University of Rochester School of Medicine and Dentistry, New York 14642, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bone Marrow Transplantation / immunology* Graft Rejection / prevention & control* Immune Tolerance* Injections Isoantigens / administration & dosage, pharmacology*, therapeutic use Kidney Transplantation / immunology*, pathology Lymphocyte Depletion Rats Rats, Inbred F344 Rats, Inbred Lew Thymus Gland Transplantation Chimera* Transplantation, Homologous |
| Grant Support | |
ID/Acronym/Agency:
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5R29-AI33054-03/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Isoantigens |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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