Document Detail


Tobacco etch virus infectivity in Capsicum spp. is determined by a maximum of three amino acids in the viral virulence determinant VPg.
MedLine Citation:
PMID:  23134519     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Potyvirus resistance in Capsicum spp. has been attributed to amino acid substitutions at the pvr1 locus that cause conformational shifts in eukaryotic translation initiation factor eIF4E. The viral genome-linked protein (VPg) sequence was isolated and compared from three Tobacco etch virus (TEV) strains, highly aphid-transmissible (HAT), Mex21, and N, which differentially infect Capsicum genotypes encoding Pvr1(+), pvr1, and pvr1(2). Viral chimeras were synthesized using the TEV-HAT genome, replacing HAT VPg with Mex21 or N VPg. TEV HAT did not infect pepper plants homozygous for either the pvr1 or pvr1(2) allele. However, the novel chimeric TEV strains, TEVHAT(Mex21-VPg) and TEV-HAT(N-VPg), infected pvr1 and pvr1(2) pepper plants, respectively, demonstrating that VPg is the virulence determinant in this pathosystem. Three dimensional structural models predicted interaction between VPg and the susceptible eIF4E genotype in every case, while resistant genotypes were never predicted to interact. To determine whether there is a correlation between physical interaction of VPg with eIF4E and infectivity, the effects of amino acid variation within VPg were assessed. Interaction between pvr1(2) eIF4E and N VPg was detected in planta, implying that the six amino acid differences in N VPg relative to HAT VPg are responsible for restoring the physical interaction and infectivity.
Authors:
Kari Perez; Inhwa Yeam; Byoung-Cheorl Kang; Daniel R Ripoll; Jinhee Kim; John F Murphy; Molly M Jahn
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Molecular plant-microbe interactions : MPMI     Volume:  25     ISSN:  0894-0282     ISO Abbreviation:  Mol. Plant Microbe Interact.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-08     Completed Date:  2013-05-06     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  9107902     Medline TA:  Mol Plant Microbe Interact     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1562-73     Citation Subset:  IM    
Affiliation:
Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY, USA.
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MeSH Terms
Descriptor/Qualifier:
Alleles
Amino Acid Sequence
Amino Acid Substitution
Capsicum / immunology,  virology*
Chimera
Disease Resistance / genetics
Eukaryotic Initiation Factor-4E / genetics*,  physiology
Genome, Viral / genetics
Host-Pathogen Interactions
Models, Molecular
Molecular Sequence Data
Plant Diseases / virology*
Plant Leaves / virology
Plant Proteins / genetics,  physiology
Potyvirus / genetics*,  pathogenicity
Protein Conformation
Protein Interaction Mapping
Sequence Alignment
Tobacco / genetics
Viral Proteins / chemistry,  genetics*,  metabolism
Virulence Factors / chemistry,  genetics*,  metabolism
Chemical
Reg. No./Substance:
0/Eukaryotic Initiation Factor-4E; 0/Plant Proteins; 0/Viral Proteins; 0/Virulence Factors
Comments/Corrections
Erratum In:
Mol Plant Microbe Interact. 2013 Apr;26(4):471

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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