Document Detail


Tn5-Mediated Cloning of a Genetic Region from Pseudomonas putida Involved in the Stimulation of Plant Root Elongation.
MedLine Citation:
PMID:  16347807     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Transposon (Tn5) mutagenesis was applied to Pseudomonas putida GR12-2R3, which promotes root elongation (a phenotype designated Pre) of Brassica campestris under gnotobiotic conditions. Of 3,000 Tn5 transconjugants, only one mutant that lost Pre activity but remained prototrophic and capable of plant root colonization was detected. This mutant was complemented by plasmid pRE53, which contained a 15.0-kilobase DNA insert isolated from a parental strain. The complemented mutant regained full Pre activity comparable to that of the wild type.
Authors:
R Lifshitz; H Guilmette; M Kozlowski
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  54     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1988 Dec 
Date Detail:
Created Date:  2010-06-25     Completed Date:  2010-06-25     Revised Date:  2010-09-20    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3169-72     Citation Subset:  -    
Affiliation:
Allelix Inc., 6850 Goreway Drive, Mississauga, Ontario L4V 1P1, Canada.
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