Document Detail


Titration and exchange studies of liver fatty acid-binding protein with 13C-labeled long-chain fatty acids.
MedLine Citation:
PMID:  11969406     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Uniformly (13)C-labeled long-chain fatty acids were used to probe ligand binding to rat liver fatty acid-binding protein (LFABP), an atypical member of the fatty acid-binding protein (FABP) family that binds more than one molecule of long-chain fatty acid, accommodates a variety of diverse ligands, and exhibits diffusion-mediated lipid transport to membranes. Two sets of (1)H-(13)C resonances were found in a titration series of NMR spectra for oleate-LFABP complexes, indicating that two molecules of the fatty acid are situated in the protein cavity. However, no distinct resonances were observed for the excess fatty acid in solution, suggesting that at least one ligand undergoes rapid exchange with oleate in the bulk solution. An exchange rate of 54 +/- 6 s(-1) between the two sets of resonances was measured directly using (13)C z,z-exchange spectroscopy. In light of these NMR measurements, possible molecular mechanisms for the ligand-exchange process are evaluated and implications for the anomalous fatty acid transport mechanism of LFABP are discussed.
Authors:
Hsin Wang; Yan He; Christopher D Kroenke; Sarala Kodukula; Judith Storch; Arthur G Palmer; Ruth E Stark
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemistry     Volume:  41     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-04-23     Completed Date:  2002-05-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5453-61     Citation Subset:  IM    
Affiliation:
Department of Chemistry, CUNY Graduate Center and College of Staten Island, Staten Island, New York 10314, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Binding Sites
Biological Transport
Carbon Isotopes / metabolism*
Carrier Proteins / chemistry*,  metabolism*
Fatty Acid-Binding Proteins
Fatty Acids / chemistry*,  metabolism*
Kinetics
Ligands
Neoplasm Proteins*
Nerve Tissue Proteins*
Nuclear Magnetic Resonance, Biomolecular / methods
Oleic Acid / chemistry,  metabolism
Palmitic Acid / chemistry,  metabolism
Protons
Radioligand Assay
Rats
Titrimetry
Grant Support
ID/Acronym/Agency:
DK38389/DK/NIDDK NIH HHS; GM59273/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 0/Carrier Proteins; 0/Fabp7 protein, rat; 0/Fatty Acid-Binding Proteins; 0/Fatty Acids; 0/Ligands; 0/Neoplasm Proteins; 0/Nerve Tissue Proteins; 0/Protons; 112-80-1/Oleic Acid; 57-10-3/Palmitic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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