| Titration and exchange studies of liver fatty acid-binding protein with 13C-labeled long-chain fatty acids. | |
| | |
MedLine Citation:
|
PMID: 11969406 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Uniformly (13)C-labeled long-chain fatty acids were used to probe ligand binding to rat liver fatty acid-binding protein (LFABP), an atypical member of the fatty acid-binding protein (FABP) family that binds more than one molecule of long-chain fatty acid, accommodates a variety of diverse ligands, and exhibits diffusion-mediated lipid transport to membranes. Two sets of (1)H-(13)C resonances were found in a titration series of NMR spectra for oleate-LFABP complexes, indicating that two molecules of the fatty acid are situated in the protein cavity. However, no distinct resonances were observed for the excess fatty acid in solution, suggesting that at least one ligand undergoes rapid exchange with oleate in the bulk solution. An exchange rate of 54 +/- 6 s(-1) between the two sets of resonances was measured directly using (13)C z,z-exchange spectroscopy. In light of these NMR measurements, possible molecular mechanisms for the ligand-exchange process are evaluated and implications for the anomalous fatty acid transport mechanism of LFABP are discussed. |
| | |
Authors:
|
Hsin Wang; Yan He; Christopher D Kroenke; Sarala Kodukula; Judith Storch; Arthur G Palmer; Ruth E Stark |
Related Documents
:
|
8742176 - Molar quantification by flow cytometry of fatty acid binding to cells using dipyrrometh... 75886 - The presence of fatty acids in human alpha-fetoprotein. 156696 - Surface mucopolysaccharides of treponema pallidum. 17427946 - Molecular definitions of fatty acid hydroxylases in arabidopsis thaliana. 12606456 - Peroxisome proliferators disrupt retinoic acid receptor alpha signaling in the testis. 11229536 - Coenzyme q10, vitamin e, and dihydrothioctic acid cooperatively prevent diene conjugati... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Biochemistry Volume: 41 ISSN: 0006-2960 ISO Abbreviation: Biochemistry Publication Date: 2002 Apr |
Date Detail:
|
Created Date: 2002-04-23 Completed Date: 2002-05-29 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: United States |
Other Details:
|
Languages: eng Pagination: 5453-61 Citation Subset: IM |
Affiliation:
|
Department of Chemistry, CUNY Graduate Center and College of Staten Island, Staten Island, New York 10314, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Binding Sites Biological Transport Carbon Isotopes / metabolism* Carrier Proteins / chemistry*, metabolism* Fatty Acid-Binding Proteins Fatty Acids / chemistry*, metabolism* Kinetics Ligands Neoplasm Proteins* Nerve Tissue Proteins* Nuclear Magnetic Resonance, Biomolecular / methods Oleic Acid / chemistry, metabolism Palmitic Acid / chemistry, metabolism Protons Radioligand Assay Rats Titrimetry |
| Grant Support | |
ID/Acronym/Agency:
|
DK38389/DK/NIDDK NIH HHS; GM59273/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Carbon Isotopes; 0/Carrier Proteins; 0/Fabp7 protein, rat; 0/Fatty Acid-Binding Proteins; 0/Fatty Acids; 0/Ligands; 0/Neoplasm Proteins; 0/Nerve Tissue Proteins; 0/Protons; 112-80-1/Oleic Acid; 57-10-3/Palmitic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Probing domain swapping for the neuronal SNARE complex with electron paramagnetic resonance.
Next Document: Mispairing of the 8,9-dihydro-8-(N7-guanyl)-9-hydroxy-aflatoxin B1 adduct with deoxyadenosine result...