| Titanium surfaces with nanotopography modulate cytokine production in cultured human gingival fibroblasts. | |
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MedLine Citation:
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PMID: 22615216 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Implant topography is an important factor that influences many cell types. To understand the role of topography in the inflammatory events, we evaluated the response of human gingival fibroblasts (HGFs) by the release pattern of cytokines. HGFs were cultured on Ti discs for 24 and 48 h. Four different surface treatments were used: machining method (turned), blasting followed by an acid-etching method (BAE), oxidative nanopatterning (ON) method, and an association of blasting followed by an acid-etching plus oxidative nanopatterning (BAE+ON) method. Extracellular levels of IL-6, IL-8, transforming growth factor beta (TGF-β), IL-4, and IL-10 were measured by enzyme-linked immunosorbant assay. Increased levels of IL-6 and IL-8 were observed in all surfaces after 24 h which decreased after 48 h. BAE, ON, and BAE+ON surfaces showed a reduction in IL-6 levels compared with the turned after 48 h (p < 0.05). On one hand, IL-8 production was lower in BAE+ON in comparison to the turned surface (p < 0.05). On the other hand, IL-4 showed increased levels with 48 h, which were significantly different between turned, BAE, and ON surfaces, but not with BAE+ON. Additionally, TGF-β and IL-10 production were not detected. This study indicates that nanotopography might be important in the modulation of the inflammatory response in cultured HGFs. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012. |
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Authors:
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Humberto Osvaldo Schwartz-Filho; Ana Carolina Faria Morandini; Erivan Schnaider Ramos-Junior; Ryo Jimbo; Carlos Ferreira Santos; Elcio Marcantonio; Ann Wennerberg; Rosemary Adriana Chiérici Marcantonio |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-5-21 |
Journal Detail:
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Title: Journal of biomedical materials research. Part A Volume: - ISSN: 1552-4965 ISO Abbreviation: - Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-5-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101234237 Medline TA: J Biomed Mater Res A Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
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Division of Periodontology, Department of Oral Diagnosis and Surgery, Araraquara School of Dentistry, UNESP, São Paulo State University, Araraquara, Brazil; Surface Biology Group, Department of Prosthodontics, Faculty of Odontology, Malmö University, Sweden. betoschwartz@hotmail.com. |
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