| Tissue-type transglutaminase from red sea bream (Pagrus major). Sequence analysis of the cDNA and functional expression in Escherichia coli. | |
| | |
MedLine Citation:
|
PMID: 7556189 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A cDNA clone encoding a tissue-type transglutaminase (TGase) was isolated from a cDNA library prepared from the liver of red sea bream (Pagrus major). The cDNA sequence had an open reading frame coding for a protein of 695 amino acids and showed 43% identity to the sequence of guinea pig liver TGase, revealing a relatively low overall similarity. However, the 25-amino-acid sequence containing the putative active site (Cys272) of the enzyme was completely conserved between the two species, and was also identical to the corresponding regions of human and bovine endothelial cell TGases. In addition, the critical residues (His332 and Asp355) thought to form the catalytic-center triad together with Cys272, were found in the highly conserved region. The red sea bream TGase had an extension of 11 amino acids in the C-terminal region and some differences in the N-terminal region when compared with guinea pig TGase. From the cloned cDNA, a semi-synthetic TGase gene suitable for overexpression in Escherichia coli was constructed (pTTG2-22). At a reduced temperature (28 degrees C), E. coli cells transformed with pTTG2-22 could produce soluble TGase which exhibited catalytic activity in the presence of calcium. E. coli extracts containing the recombinant red sea bream TGase induced gelation of actomyosin solutions, accompanied by a significant increase of epsilon-(gamma-glutamyl)lysine bonds, which are predominantly derived from the cross-linking of myosin heavy chains. These results indicate that this fish TGase should be useful for further analysis of TGase structure/function relationships and that it could also be employed to enhance the viscoelastic properties of proteinaceous materials. |
| | |
Authors:
|
H Yasueda; K Nakanishi; Y Kumazawa; K Nagase; M Motoki; H Matsui |
Related Documents
:
|
6328279 - Drosophila melanogaster has only one myosin alkali light-chain gene which encodes a pro... 9254009 - Cloning and characterization of novel gene, dcrr1, expressed from down's syndrome criti... 8875889 - Swine cytosolic malic enzyme: cdna cloning, sequencing, and localization. 1998509 - A unique cellular myosin ii exhibiting differential expression in the cerebral cortex. 2148519 - Construction, expression and unexpected regulatory properties of a tropomyosin mutant w... 9367679 - Characterization of the gene encoding human sarcolipin (sln), a proteolipid associated ... 21705609 - Genome sequence of leuconostoc pseudomesenteroides kctc 3652. 11274109 - Regulation of the acetoin catabolic pathway is controlled by sigma l in bacillus subtilis. 21622329 - Significance of ecological vicariance and long-distance dispersal in the diversificatio... |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: European journal of biochemistry / FEBS Volume: 232 ISSN: 0014-2956 ISO Abbreviation: Eur. J. Biochem. Publication Date: 1995 Sep |
Date Detail:
|
Created Date: 1995-11-14 Completed Date: 1995-11-14 Revised Date: 2007-07-23 |
Medline Journal Info:
|
Nlm Unique ID: 0107600 Medline TA: Eur J Biochem Country: GERMANY |
Other Details:
|
Languages: eng Pagination: 411-9 Citation Subset: IM |
Affiliation:
|
Food Research & Development Laboratories, Ajinomoto Co., Inc., Japan. |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GENBANK/S79761 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Actomyosin
/
metabolism Amino Acid Sequence Animals Base Sequence Cattle DNA, Complementary / genetics* Escherichia coli / genetics Fishes / genetics*, metabolism Gene Expression Guinea Pigs Humans Liver / enzymology Molecular Sequence Data Recombinant Proteins / genetics, metabolism Restriction Mapping Sequence Homology, Amino Acid Species Specificity Transglutaminases / genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Complementary; 0/Recombinant Proteins; 9013-26-7/Actomyosin; EC 2.3.2.13/Transglutaminases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Antisense effects of cholesterol-oligodeoxynucleotide conjugates associated with poly(alkylcyanoacry...
Next Document: Na+/K(+)-ATPase with a blocked E1ATP site still allows backdoor phosphorylation of the E2ATP site.