|Tissue specific variants of glutamate transporter GLT-1.|
|PMID: 9373176 Owner: NLM Status: MEDLINE|
|cDNA cloning of glutamate transporter GLT-1 from mouse brain and liver has revealed that 5'-ends of the messages are different between brain and liver. In addition, one of the GLT-1 cDNAs isolated from liver has been found to possess a 3'-end different from those of the others. Reverse transcription polymerase chain reaction (RT-PCR) amplification using primers specific for altered 5'-ends has confirmed that brain and liver messages possess their own specific 5'-ends. Both of the two 3'-ends have been demonstrated by RT-PCR to be present not only in liver but also in brain, indicating both brain and liver GLT-1 possess two types of 3'-ends. Although functional properties are not changed by the alteration of N-termini and C-termini when expressed in Xenopus laevis oocytes, co-expression of two liver type GLT-1 with different C-termini (mGLT-1A and mGLT-1B) has been found to result in the increase in Vmax of transport without changing Km. These results suggest the tissue specific alternative splicing at 5'-ends of GLT-1 messages and the interesting association of spliced variants with different C-termini.|
|N Utsunomiya-Tate; H Endou; Y Kanai|
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|Type: Journal Article; Research Support, Non-U.S. Gov't|
|Title: FEBS letters Volume: 416 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1997 Oct|
|Created Date: 1997-12-08 Completed Date: 1997-12-08 Revised Date: 2008-11-21|
Medline Journal Info:
|Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS|
|Languages: eng Pagination: 312-6 Citation Subset: IM|
|Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Mitaka, Tokyo, Japan.|
|Data Bank Information|
Bank Name/Acc. No.:
|GENBANK/AB007810; AB007811; AB007812|
|APA/MLA Format Download EndNote Download BibTex|
ATP-Binding Cassette Transporters
Amino Acid Sequence
Amino Acid Transport System X-AG
Brain / metabolism
Glutamic Acid / metabolism
Liver / metabolism
Molecular Sequence Data
Oocytes / physiology
Polymerase Chain Reaction
RNA, Messenger / biosynthesis
Recombinant Proteins / chemistry, metabolism
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
|0/ATP-Binding Cassette Transporters; 0/Amino Acid Transport System X-AG; 0/DNA Primers; 0/RNA, Messenger; 0/Recombinant Proteins; 56-86-0/Glutamic Acid|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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