Document Detail

Tissue specific variants of glutamate transporter GLT-1.
MedLine Citation:
PMID:  9373176     Owner:  NLM     Status:  MEDLINE    
cDNA cloning of glutamate transporter GLT-1 from mouse brain and liver has revealed that 5'-ends of the messages are different between brain and liver. In addition, one of the GLT-1 cDNAs isolated from liver has been found to possess a 3'-end different from those of the others. Reverse transcription polymerase chain reaction (RT-PCR) amplification using primers specific for altered 5'-ends has confirmed that brain and liver messages possess their own specific 5'-ends. Both of the two 3'-ends have been demonstrated by RT-PCR to be present not only in liver but also in brain, indicating both brain and liver GLT-1 possess two types of 3'-ends. Although functional properties are not changed by the alteration of N-termini and C-termini when expressed in Xenopus laevis oocytes, co-expression of two liver type GLT-1 with different C-termini (mGLT-1A and mGLT-1B) has been found to result in the increase in Vmax of transport without changing Km. These results suggest the tissue specific alternative splicing at 5'-ends of GLT-1 messages and the interesting association of spliced variants with different C-termini.
N Utsunomiya-Tate; H Endou; Y Kanai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  416     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1997 Oct 
Date Detail:
Created Date:  1997-12-08     Completed Date:  1997-12-08     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  312-6     Citation Subset:  IM    
Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Mitaka, Tokyo, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB007810;  AB007811;  AB007812
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MeSH Terms
ATP-Binding Cassette Transporters / biosynthesis*,  chemistry,  physiology
Alternative Splicing*
Amino Acid Sequence
Amino Acid Transport System X-AG
Base Sequence
Brain / metabolism
Cloning, Molecular
DNA Primers
Genetic Variation*
Glutamic Acid / metabolism
Liver / metabolism
Molecular Sequence Data
Oocytes / physiology
Organ Specificity
Polymerase Chain Reaction
RNA, Messenger / biosynthesis
Recombinant Proteins / chemistry,  metabolism
Sequence Alignment
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Xenopus laevis
Reg. No./Substance:
0/ATP-Binding Cassette Transporters; 0/Amino Acid Transport System X-AG; 0/DNA Primers; 0/RNA, Messenger; 0/Recombinant Proteins; 56-86-0/Glutamic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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