Document Detail

Tissue-specific induction and inactivation of cytochrome P450 catalysing lauric acid hydroxylation in the sea bass, Dicentrarchus labrax.
MedLine Citation:
PMID:  10327614     Owner:  NLM     Status:  MEDLINE    
Microsomal cytochrome P450-dependent lauric acid hydroxylase activities were characterized in liver, kidney, and intestinal mucosa of the sea bass (Dicentrarchus labrax). Microsomes from these organs generated (omega-1)-hydroxylauric acid and a mixture of positional isomers including (omega)-, (omega-2)-, (omega-3)- and (omega-4)-hydroxylauric acids, which were identified by RP-HPLC and GC-MS analysis. Peroxisome proliferators, such as clofibrate and especially di(2-ethylhexyl) phthalate, increased kidney microsomal lauric acid hydroxylase activities. The synthesis of 11-hydroxylauric acid was enhanced 5.3-fold in kidney microsomes. Liver microsomal lauric acid hydroxylase activities were weakly affected and no significant induction was found in small intestine microsomes from clofibrate or di(2-ethylhexyl) phthalate-treated fish. The differences in lauric acid metabolisation and the tissue-specific induction by peroxisome proliferators suggest the involvement of several P450s in this reaction. Incubations of liver and kidney microsomes with lauric acid analogues (11- or 10-dodecynoic acids) resulted in a time- and concentration-dependent loss of lauric acid hydroxylase activities. The induction of these activities in fish by phthalates, which are widely-distributed environmental pollutants, may be taken into consideration for the development of new biomarkers.
C Sabourault; G de Sousa; M Amichot; A Cuany; R Rahmani; J P Salaün; J B Bergé; J P Girard; M Lafaurie
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology     Volume:  122     ISSN:  1096-4959     ISO Abbreviation:  Comp. Biochem. Physiol. B, Biochem. Mol. Biol.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-06-28     Completed Date:  1999-06-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9516061     Medline TA:  Comp Biochem Physiol B Biochem Mol Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  253-60     Citation Subset:  IM    
Université de Nice-Sophia Antipolis, Laboratoire de Physiologie et Toxicologie Environnementales, Faculté des Sciences, France.
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MeSH Terms
Bass / metabolism*
Biological Markers
Clofibrate / pharmacology
Cytochrome P-450 Enzyme System / drug effects,  metabolism*
Diethylhexyl Phthalate / pharmacology
Fatty Acids, Unsaturated / metabolism,  pharmacology
Intestine, Small / drug effects,  metabolism
Kidney / drug effects,  metabolism
Lauric Acids / metabolism*
Liver / drug effects,  metabolism
Microsomes / drug effects,  metabolism
Organ Specificity
Peroxisome Proliferators / pharmacology*
Substrate Specificity
Reg. No./Substance:
0/Biological Markers; 0/Fatty Acids, Unsaturated; 0/Lauric Acids; 0/Peroxisome Proliferators; 117-81-7/Diethylhexyl Phthalate; 143-07-7/lauric acid; 16900-60-0/11-dodecynoic acid; 637-07-0/Clofibrate; 9035-51-2/Cytochrome P-450 Enzyme System

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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