Document Detail


Tissue-specific function of Patj in regulating the Crumbs complex and epithelial polarity.
MedLine Citation:
PMID:  23136386     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Patj is described as a core component of the Crumbs complex. Along with the other components, Crumbs and Stardust, Patj has been proposed as essential for epithelial polarity. However, no proper in vivo genetic analysis of Patj function has been performed in any organism. We have generated the first null mutants for Drosophila Patj. These mutants are lethal. However, Patj is not required in all epithelia where the Crumbs complex is essential. Patj is dispensable for ectoderm polarity and embryonic development, whereas more severe defects are observed in the adult follicular epithelium, including mislocalisation of the Crumbs complex from the apical domain, as well as morphogenetic defects. These defects are similar to those observed with crumbs and stardust mutants, although weaker and less frequent. Also, gain-of-function of Crumbs and Patj mutation genetically suppress each other in follicular cells. We also show that the first PDZ domain of Patj associated with the Stardust-binding domain are sufficient to fully rescue both Drosophila viability and Crumbs localisation. We propose that the only crucial function of Patj hinges on the ability of its first two domains to positively regulate the Crumbs complex, defining a new developmental level of regulation of its dynamics.
Authors:
Clothilde Pénalva; Vincent Mirouse
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-7
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  -     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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