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Tissue specific expression of the rat Ah-receptor and ARNT mRNAs.
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MedLine Citation:
PMID:  8065918     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Ah-receptor (AHR) is a ligand activated transcription factor that mediates the biological effects of agonists such as 2,3,7,8-tetrachlorodibenzo-p-dioxin. Upon binding agonists, the AHR dimerizes with a structurally related protein known as ARNT and this heterodimer then binds cognate enhancer elements and activates the expression of target genes. In this report we describe the cloning of the rat AHR cDNA and a fragment of the rat ARNT cDNA for use as probes in ribonuclease protection analysis. Ribonuclease protection analysis indicated that the rat AHR mRNA is expressed at the highest levels in the lung > thymus > kidney > liver while lower levels were expressed in heart and spleen. The rat AHR and ARNT mRNAs were expressed in a largely coordinate manner across the eight tissues examined with the exception of the placenta where AHR levels were relatively low compared to ARNT. In these experiments, a rare splice variant of the AHR was cloned that encoded a protein with a deletion in the ligand binding domain. In vitro expression studies demonstrated that in contrast to the full length AHR, the splice variant did not bind ligand nor did it bind to a cognate enhancer element in the presence of ARNT.
Authors:
L A Carver; J B Hogenesch; C A Bradfield
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Nucleic acids research     Volume:  22     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1994 Aug 
Date Detail:
Created Date:  1994-09-21     Completed Date:  1994-09-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  3038-44     Citation Subset:  IM    
Affiliation:
Department of Molecular Pharmacology and Biological Chemistry, Northwestern University Medical School, Chicago, IL 60611.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/U08986;  U09000
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Aryl Hydrocarbon Receptor Nuclear Translocator
Base Sequence
Cloning, Molecular
DNA Probes
DNA-Binding Proteins*
Enhancer Elements, Genetic
Female
Gene Expression*
Humans
Macromolecular Substances
Molecular Sequence Data
Proteins / chemistry,  genetics*
RNA, Messenger / metabolism*
Rats
Rats, Inbred F344
Rats, Sprague-Dawley
Receptors, Aryl Hydrocarbon / chemistry,  genetics*
Restriction Mapping
Ribonucleases / metabolism
Tissue Distribution
Transcription Factors*
Grant Support
ID/Acronym/Agency:
ES-5703/ES/NIEHS NIH HHS; T32-GM08061/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/ARNT protein, human; 0/ARNT protein, rat; 0/DNA Probes; 0/DNA-Binding Proteins; 0/Macromolecular Substances; 0/Proteins; 0/RNA, Messenger; 0/Receptors, Aryl Hydrocarbon; 0/Transcription Factors; 138391-32-9/Aryl Hydrocarbon Receptor Nuclear Translocator; EC 3.1.-/Ribonucleases
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 11 Month: 8 Year: 1994
Volume: 22 Issue: 15
First Page: 3038 Last Page: 3044
ID: 310273
PubMed Id: 8065918

Tissue specific expression of the rat Ah-receptor and ARNT mRNAs.
L A Carver
J B Hogenesch
C A Bradfield
Department of Molecular Pharmacology and Biological Chemistry, Northwestern University Medical School, Chicago, IL 60611.



Article Categories:
  • Research Article


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