Document Detail


Tissue-specific and age-related variations in repetitive sequences of mouse extrachromosomal circular DNAs.
MedLine Citation:
PMID:  2320037     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Extrachromosomal circular (ecc) DNA was isolated from mouse brain, liver, and heart tissues at different ages. To determine the abundance of repetitive sequences in eccDNAs, preparations were probed for short-interspersed (B1 and B2), long-interspersed (L1), endogenous retroviral-like (IAP), and tandemly repeated satellite sequences (SAT) of the mouse genome. Together these sequence families comprise approximately 15% of the mouse genome. The hybridization results showed that each tissue had a characteristic pattern of repetitive sequence elements in eccDNAs, and the abundance of repetitive sequences changed as a function of age. Repetitive sequences decreased in liver and brain eccDNAs from 1 month to 8 months of age but appeared to remain stable thereafter. In contrast, repetitive sequence families in heart eccDNAs were constant from 1 month to 16 months of age but declined in 24-month-old mice. The present studies indicate that extrachromosomal sequences exhibit greater flexibility than chromosomal sequences.
Authors:
J W Gaubatz; S C Flores
Related Documents :
10756097 - The structure and expression of the murine wildtype p53-induced phosphatase 1 (wip1) gene.
11718897 - Molecular characterisation of mouse and human tssc6: evidence that tssc6 is a genuine m...
11252167 - Molecular cloning, genetic mapping, and expression of the mouse sf3b1 (sap155) gene for...
1846947 - A maternally inherited superantigen encoded by a mammary tumour virus.
14687567 - Methionine adenosyltransferase as a useful molecular systematics tool revealed by phylo...
21428987 - Ribosomal rna-based profiling of bacteria in the axilla of healthy males suggests right...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Mutation research     Volume:  237     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-05-10     Completed Date:  1990-05-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  29-36     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of South Alabama, College of Medicine, Mobile 36688.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Aging / genetics*
Animals
Brain / metabolism
DNA Probes
DNA, Circular / genetics*,  isolation & purification
DNA, Satellite / genetics*,  isolation & purification
Immunoblotting
Liver / metabolism
Male
Mice
Mice, Inbred C57BL
Myocardium / metabolism
Repetitive Sequences, Nucleic Acid / genetics*
Chemical
Reg. No./Substance:
0/DNA Probes; 0/DNA, Circular; 0/DNA, Satellite

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Age-dependent covalent DNA alterations (I-compounds) in rat liver mitochondrial DNA.
Next Document:  DNA alterations in prostatic adenocarcinoma and benign prostatic hyperplasia: detection by DNA finge...