Document Detail


Tissue and serum loss of metalloproteinase inhibitors in high grade soft tissue sarcomas.
MedLine Citation:
PMID:  12973672     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The activity of matrix metalloproteinases (MMPs) in degrading extracellular matrix is controlled by activation of pro-enzymes and inhibition of MMP tissue inhibitors (TIMPs). To assess proteolytic cascade imbalance in malignancy progression, the enzymatic activity of MMP2 and MMP9 and the expression and serum level of their inhibitors, TIMP2 and TIMP1 respectively, was evaluated in selected patients with high-risk soft tissue sarcoma (STS). Gelatinase activity and inhibitor expression was evaluated on 69 biopsies by zymography and immunohistochemistry. TIMP1 and TIMP2 serum concentration was tested in 53 STS patients and in 56 controls using a sandwich enzyme immunoassay. Clinical and biological variables were related to clinical outcome of the patients. A significant gelatinolytic activity was seen in a high percentage of STS. TIMP expression was weak or negative in the majority of samples. The difference between disease-free (p=0.001) and overall survival (p=0.007) curves based on TIMP2 immunoreactivity was statistically significant. TIMP plasma concentration of 53 STS revealed significantly lower levels compared to those of 56 controls (p=0.0001). In conclusion, low levels of negative regulators of proteolysis may be related to tumor biological aggressiveness and used to select patients with poor prognosis to improve cure.
Authors:
M S Benassi; G Magagnoli; F Ponticelli; L Pazzaglia; L Zanella; G Gamberi; P Ragazzini; C Ferrari; M Mercuri; P Picci
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Histology and histopathology     Volume:  18     ISSN:  0213-3911     ISO Abbreviation:  Histol. Histopathol.     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-09-15     Completed Date:  2004-06-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8609357     Medline TA:  Histol Histopathol     Country:  Spain    
Other Details:
Languages:  eng     Pagination:  1035-40     Citation Subset:  IM    
Affiliation:
Oncological Research Laboratory, Istituti Ortopedici Rizzoli, Bologna, Italy. mariaserena.benassi@ior.it
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Aged, 80 and over
Child
Disease Progression
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunohistochemistry
Male
Matrix Metalloproteinase 2 / biosynthesis
Matrix Metalloproteinase 9 / biosynthesis
Middle Aged
Neoplasm Metastasis / pathology
Sarcoma / enzymology*,  mortality,  pathology
Soft Tissue Neoplasms / enzymology*,  mortality,  pathology
Survival Analysis
Tissue Inhibitor of Metalloproteinase-1 / blood,  metabolism
Tissue Inhibitor of Metalloproteinase-2 / blood,  metabolism
Tissue Inhibitor of Metalloproteinases / blood,  metabolism*
Chemical
Reg. No./Substance:
0/Tissue Inhibitor of Metalloproteinase-1; 0/Tissue Inhibitor of Metalloproteinases; 127497-59-0/Tissue Inhibitor of Metalloproteinase-2; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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