Document Detail

Tissue reactions to epoxy-crosslinked porcine heart valves post-treated with detergents or a dicarboxylic acid.
MedLine Citation:
PMID:  11255196     Owner:  NLM     Status:  MEDLINE    
Calcification limits the long-term durability of xenograft glutaraldehyde (GA)-crosslinked heart valves. Previously, a study in rats showed that epoxy-crosslinked heart valves reduced lymphocyte reactions to the same extent as the GA-crosslinked control and induced a similar foreign-body response and calcification reaction. The present study was aimed at reducing the occurrence of calcification of epoxy-crosslinked tissue. Two modifications were carried out and their influence on cellular reactions and the extent of calcification after 8 weeks' implantation in weanling rats was evaluated. First, epoxy-crosslinked valves were post-treated with two detergents to remove cellular elements, phospholipids and small soluble proteins, known to act as nucleation sites for calcification. The second approach was to study the effect of the impaired balance between negatively and positively charged amino acids by an additional crosslinking step with a dicarboxylic acid. The detergent treatment resulted in a washed-out appearance of especially the cusp tissue. With the dicarboxylic acid, both the cusps and the walls had a limited washed-out appearance. The wall also demonstrated some detachment of the subendothelium. After implantation, both detergent and dicarboxylic acid post-treatment histologically resulted in reduced calcification at the edges of cusps and walls. However, total amounts of calcification, measured by atomic emission spectroscopy, were not significantly reduced. Data concerning the presence of lymphocytes varied slightly, but were in the same range as the GA-crosslinked control, i.e., clearly reduced compared with a noncrosslinked control. It is concluded that both the double detergent and the dicarboxylic acid post-treatment of epoxy-crosslinked heart valve tissue do not represent a sound alternative in the fabrication of heart valve bioprostheses.
P B van Wachem; L A Brouwer; R Zeeman; P J Dijkstra; J Feijen; M Hendriks; P T Cahalan; M J van Luyn
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Journal of biomedical materials research     Volume:  55     ISSN:  0021-9304     ISO Abbreviation:  J. Biomed. Mater. Res.     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-03-20     Completed Date:  2001-05-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0112726     Medline TA:  J Biomed Mater Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  415-23     Citation Subset:  IM    
Copyright Information:
Copyright 2001 John Wiley & Sons, Inc. J Biomed Mater Res 55: 415-423, 2001
University of Groningen, Medical Biology, Tissue Engineering, University Hospital, Entrance 25, Hanzeplein 1, 9713 GZ Groningen, The Netherlands.
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MeSH Terms
Butylene Glycols
Calcinosis / pathology,  prevention & control
Cross-Linking Reagents
Heart Valve Prosthesis*
Materials Testing
Transplantation, Heterologous
Reg. No./Substance:
0/Butylene Glycols; 0/Carbodiimides; 0/Cross-Linking Reagents; 0/Detergents; 111-30-8/Glutaral; 2425-79-8/1,4-bis(2,3-epoxypropoxy)butane

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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