| Tissue kallikrein and kinin infusion promotes neovascularization in limb ischemia. | |
| | |
MedLine Citation:
|
PMID: 18627294 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Adenovirus-mediated kallikrein delivery has been shown to promote blood vessel growth in the limb under both ischemic and normoperfused conditions. Here we investigated whether a continuous supply of kallikrein and kinin peptide can induce neovascularization in a rat model of hindlimb ischemia. Rats underwent femoral artery ligation and localized injection of tissue kallikrein, bradykinin or B1 receptor agonist, followed by infusion of proteins by osmotic minipump. Regional blood flow was monitored weekly by laser Doppler perfusion imaging. Three weeks after surgery, rats receiving kallikrein and kinins showed a significant increase in the perfusion ratio of ischemic vs. normoperfused limb compared to control rats. Similarly, a microsphere assay showed that kallikrein and kinins significantly increased regional blood flow without altering blood pressure. Moreover, kallikrein and kinins significantly augmented capillary and arteriole densities, as quantified by immunostaining with CD-31 and smooth muscle alpha-actin. Both tissue kallikrein and bradykinin increased hemoglobin content in Matrigel implants in mice, providing further evidence of the angiogenic properties. Kinins, when delivered subcutaneously via Matrigel in rats, also increased regional perfusion. This is the first demonstration that local application of tissue kallikrein protein or kinin peptide has therapeutic value in the treatment of ischemic disease by promoting neovascularization. |
| | |
Authors:
|
Robert S Smith; Lin Gao; Lee Chao; Julie Chao |
Related Documents
:
|
4079444 - Perfusion of the isolated rat hind limb. an analysis of the technique. 3934984 - Ammonia and bicarbonate transport by rat cortical collecting ducts perfused in vitro. 16391174 - Enhanced endothelin synthesis by endothelial cells exposed to sera from pregnant rats w... 3480354 - Indomethacin antagonizes furosemide's intratubular effects during loop segment microper... 9113274 - Chronic ethanol downregulates gal-beta-1,4glcnac alpha 2,6-sialyltransferase and gal-be... 6835714 - L-proline transport by isolated renal tubules from newborn and adult rats. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
|
Title: Biological chemistry Volume: 389 ISSN: 1431-6730 ISO Abbreviation: Biol. Chem. Publication Date: 2008 Jun |
Date Detail:
|
Created Date: 2008-09-04 Completed Date: 2008-09-26 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9700112 Medline TA: Biol Chem Country: Germany |
Other Details:
|
Languages: eng Pagination: 725-30 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Arterioles / growth & development Capillaries / growth & development Collagen Drug Combinations Hemoglobins / metabolism Hindlimb / blood supply*, drug effects, physiopathology* Injections Ischemia / physiopathology* Kinins / administration & dosage, pharmacology* Laminin Lasers Male Microspheres Neovascularization, Physiologic / drug effects* Peptides / administration & dosage, pharmacology Perfusion Proteoglycans Rats Receptor, Bradykinin B1 / antagonists & inhibitors Receptor, Bradykinin B2 / antagonists & inhibitors Tissue Kallikreins / administration & dosage, pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
|
C06 RR015455/RR/NCRR NIH HHS; DK-066250/DK/NIDDK NIH HHS; HL-29373/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Drug Combinations; 0/Hemoglobins; 0/Kinins; 0/Laminin; 0/Peptides; 0/Proteoglycans; 0/Receptor, Bradykinin B1; 0/Receptor, Bradykinin B2; 119978-18-6/matrigel; 9007-34-5/Collagen; EC 3.4.21.35/Tissue Kallikreins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Attenuation of left ventricular dysfunction by an ACE inhibitor after myocardial infarction in a kin...
Next Document: Doxorubicin cardiomyopathy-induced inflammation and apoptosis are attenuated by gene deletion of the...