| Tissue immunoassay for 19F-tagged 5-hydroxytryptophan. | |
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MedLine Citation:
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PMID: 14677652 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new tool for magnetic resonance, L-6-heptafluorobutyryl-5-hydroxytryptophan, was synthesized and investigated using an antibody to perfluoroalkyl moieties developed previously. To be useful as an imaging agent, the compound must cross the blood brain barrier and then be concentrated in vesicles in serotonergic neurons in order to accumulate in sufficient quantity for in vivo detection to be possible. The novel imaging compound was administered in ova to domestic chicks (Gallus domestics) to investigate the bioavailability and uptake dynamics of the compound in this model organism. Typical immunoassay methods were ineffective, so a new technique was developed which binds amines and amino acids to the walls of acid-functionalized cuvettes. The first study established the presence of higher quantities of the tags in neural and liver tissue than in heart tissue. A second study investigated regional differences, with the midbrain containing more tagged compounds than the frontal lobe sample, and the frontal lobe sample containing more than the occipital or cerebellum samples. These studies demonstrate that the compound follows the pathway of endogenous serotonin. A third study investigated uptake dynamics of the novel compound. Maximum concentration of the tagged molecule in the brain was achieved three days after injecting Incubation Day 14 eggs, suggesting that it bioaccumulates in vivo. This new immunoassay technique used to detect the novel compound in tissue samples demonstrated good repeatability. |
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Authors:
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Sherry Dingman; Lara Hurlburt; Rhys Thomas; Congyuan Guo |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of immunoassay & immunochemistry Volume: 24 ISSN: 1532-1819 ISO Abbreviation: J Immunoassay Immunochem Publication Date: 2003 |
Date Detail:
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Created Date: 2003-12-17 Completed Date: 2004-08-09 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 100963688 Medline TA: J Immunoassay Immunochem Country: United States |
Other Details:
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Languages: eng Pagination: 325-44 Citation Subset: IM |
Affiliation:
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Marist College, Poughkeepsie, New York 12601, USA. Sherry.Dingman@marist.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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5-Hydroxytryptophan
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analogs & derivatives,
analysis,
chemical synthesis,
pharmacokinetics* Animals Blood-Brain Barrier / embryology, metabolism Brain Chemistry* Cerebellum / embryology, metabolism Chick Embryo / metabolism* Fluorine Fluorocarbons / analysis, chemical synthesis, pharmacokinetics Frontal Lobe / embryology, metabolism Immunoassay / methods* Isotopes Liver / embryology, metabolism Magnetic Resonance Spectroscopy* Mesencephalon / embryology, metabolism Serotonin / chemistry, metabolism Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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1R15DA013413-01/DA/NIDA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/6-heptafluorobutyryl-5-hydroxytryptophan; 0/Fluorocarbons; 0/Isotopes; 50-67-9/Serotonin; 56-69-9/5-Hydroxytryptophan; 7782-41-4/Fluorine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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