Document Detail


Tissue factor production by cultured rat mesangial cells. Stimulation by TNF alpha and lipopolysaccharide.
MedLine Citation:
PMID:  2345426     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fibrin formation plays an important role in glomerular injury. We therefore examined the procoagulant signal produced by cultured rat mesangial cells. Actively growing mesangial cells produced procoagulant activity (PCA) that was present in intact cells (surface-associated), was inhibitable by cyclohexamide and which, by clotting assay, had the characteristics of tissue factor. This PCA decreased with incubation of cells in serum-deprived medium. Incubation with bacterial lipopolysaccharide (LPS) and tumor necrosis factor (TNF alpha) induced increased detectable tissue factor by mesangial cells within two hours which was maximal by four hours. We conclude that quiescent mesangial cells produce a small amount of tissue factor-like procoagulant activity, and that this PCA can be stimulated by incubation with TNF alpha, LPS or when cells are actively growing in high serum medium. Therefore mesangial cells have the capability of contributing to fibrin formation during inflammatory glomerular injury or sepsis.
Authors:
R C Wiggins; N Njoku; J R Sedor
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Kidney international     Volume:  37     ISSN:  0085-2538     ISO Abbreviation:  Kidney Int.     Publication Date:  1990 May 
Date Detail:
Created Date:  1990-06-29     Completed Date:  1990-06-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0323470     Medline TA:  Kidney Int     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1281-5     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, University of Michigan Medical School, Ann Arbor.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Coagulation Factors / biosynthesis*
Cells, Cultured
Glomerular Mesangium / metabolism*
Interleukin-1 / pharmacology
Lipopolysaccharides / pharmacology*
Monocytes / metabolism
Rats
Rats, Inbred Strains
Tumor Necrosis Factor-alpha / pharmacology*
Grant Support
ID/Acronym/Agency:
AM30673/AM/NIADDK NIH HHS; DK38149/DK/NIDDK NIH HHS; DK39255/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Blood Coagulation Factors; 0/Interleukin-1; 0/Lipopolysaccharides; 0/Tumor Necrosis Factor-alpha

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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