| Tissue factor production by cultured rat mesangial cells. Stimulation by TNF alpha and lipopolysaccharide. | |
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MedLine Citation:
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PMID: 2345426 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fibrin formation plays an important role in glomerular injury. We therefore examined the procoagulant signal produced by cultured rat mesangial cells. Actively growing mesangial cells produced procoagulant activity (PCA) that was present in intact cells (surface-associated), was inhibitable by cyclohexamide and which, by clotting assay, had the characteristics of tissue factor. This PCA decreased with incubation of cells in serum-deprived medium. Incubation with bacterial lipopolysaccharide (LPS) and tumor necrosis factor (TNF alpha) induced increased detectable tissue factor by mesangial cells within two hours which was maximal by four hours. We conclude that quiescent mesangial cells produce a small amount of tissue factor-like procoagulant activity, and that this PCA can be stimulated by incubation with TNF alpha, LPS or when cells are actively growing in high serum medium. Therefore mesangial cells have the capability of contributing to fibrin formation during inflammatory glomerular injury or sepsis. |
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Authors:
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R C Wiggins; N Njoku; J R Sedor |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Kidney international Volume: 37 ISSN: 0085-2538 ISO Abbreviation: Kidney Int. Publication Date: 1990 May |
Date Detail:
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Created Date: 1990-06-29 Completed Date: 1990-06-29 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0323470 Medline TA: Kidney Int Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1281-5 Citation Subset: IM |
Affiliation:
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Department of Internal Medicine, University of Michigan Medical School, Ann Arbor. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Coagulation Factors / biosynthesis* Cells, Cultured Glomerular Mesangium / metabolism* Interleukin-1 / pharmacology Lipopolysaccharides / pharmacology* Monocytes / metabolism Rats Rats, Inbred Strains Tumor Necrosis Factor-alpha / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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AM30673/AM/NIADDK NIH HHS; DK38149/DK/NIDDK NIH HHS; DK39255/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Blood Coagulation Factors; 0/Interleukin-1; 0/Lipopolysaccharides; 0/Tumor Necrosis Factor-alpha |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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