| Tissue factor activity of syncytiotrophoblast plasma membranes and tumoral trophoblast cells in culture. | |
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MedLine Citation:
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PMID: 7740495 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During pregnancy, important modifications of hemostasis occur resulting in mothers in hypercoagulability and the role of placental cells such as trophoblast cells has been hypothesized. In this study, we first showed that syncytiotrophoblast plasma membranes, isolated from normal human placenta, expressed a strong tissue factor (TF) activity. We then studied TF activity of two continuous trophoblast cell lines (JEG-3 and BeWo) in comparison to human umbilical vein endothelial cells (HUVEC) and transformed human endothelial cells (ECV-304). TF assays were performed on intact detached confluent cells. Unstimulated JEG-3 and BeWo cells exhibited a very high TF activity which slightly increased after 2 to 4 h TNF-alpha stimulation. In contrast, HUVEC and ECV-304 had a lower basal TF activity which was mainly inducible by TNF-alpha, with a maximum effect after 4 to 6 h stimulation. For both cell types, TF activity was decreased to basal value after 16-hour TNF-alpha stimulation. These results support that trophoblast cells are able to express TF but the involvement of this property in the hemostatic physiological changes observed during pregnancy, remains to be demonstrated. |
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Authors:
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P Reverdiau; A C Jarousseau; G Thibault; B Khalfoun; H Watier; Y Lebranchu; P Bardos; Y Gruel |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Thrombosis and haemostasis Volume: 73 ISSN: 0340-6245 ISO Abbreviation: Thromb. Haemost. Publication Date: 1995 Jan |
Date Detail:
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Created Date: 1995-06-05 Completed Date: 1995-06-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7608063 Medline TA: Thromb Haemost Country: GERMANY |
Other Details:
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Languages: eng Pagination: 49-54 Citation Subset: IM |
Affiliation:
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Groupe Interactions Hôte-Greffon, Laboratories d'Hématologie et d'Immunologie, Faculté de Médecine, Université François Rabelais, Tours, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line, Transformed
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drug effects Cell Membrane / chemistry* Cells, Cultured / drug effects Choriocarcinoma / chemistry*, pathology Cycloheximide / pharmacology Dactinomycin / pharmacology Endothelium, Vascular / chemistry, drug effects Female Gene Expression Regulation / drug effects Gene Expression Regulation, Neoplastic / drug effects Humans Neoplasm Proteins / analysis*, biosynthesis, genetics Pregnancy / blood* Thromboplastin / analysis*, biosynthesis, genetics Trophoblasts / chemistry* Tumor Cells, Cultured / drug effects Tumor Necrosis Factor-alpha / pharmacology Umbilical Veins Uterine Neoplasms / chemistry*, pathology |
| Chemical | |
Reg. No./Substance:
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0/Neoplasm Proteins; 0/Tumor Necrosis Factor-alpha; 50-76-0/Dactinomycin; 66-81-9/Cycloheximide; 9035-58-9/Thromboplastin |
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