Document Detail


Tissue factor activity in HeLa cells measured with a continuous chromogenic assay and ELISA reader.
MedLine Citation:
PMID:  3515617     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tissue factor activity expressed by Hela cells cultured in 96-well plates has been quantitated in situ using a continuous spectrophotometric assay. Following the assay, cells assayed without physical disruption remained as viable as cells not subjected to the assay. Very little (or no) tissue factor was expressed in nondisrupted cells relative to that available in cells disrupted by freeze-thawing and sonication. Total tissue factor activity (that available in disrupted cells) decreased not as a simple function of time after subculturing, but was inversely related to cell density.
Authors:
S D Carson; P G Archer
Related Documents :
20032407 - In vitro effects of dichloroacetate and co2 on hypoxic hela cells.
16326057 - Methanolic extract of pterocarpus santalinus induces apoptosis in hela cells.
1336207 - Herpes simplex virus induces unscheduled dna synthesis in virus-infected cervical cance...
16457587 - Gene-specific characterization of human histone h2b by electron capture dissociation.
20067997 - Fak-mediated extracellular signals are essential for interkinetic nuclear migration and...
20396067 - Windowless cell system for luminescence studies on liquids under excitation in the vuv.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Thrombosis research     Volume:  41     ISSN:  0049-3848     ISO Abbreviation:  Thromb. Res.     Publication Date:  1986 Jan 
Date Detail:
Created Date:  1986-04-28     Completed Date:  1986-04-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0326377     Medline TA:  Thromb Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  185-95     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Cytological Techniques
Enzyme-Linked Immunosorbent Assay
Factor VII / physiology
Factor VIIa
Factor X / physiology
Hela Cells / metabolism*
Humans
Methods
Models, Biological
Spectrophotometry
Thromboplastin / metabolism*
Time Factors
Grant Support
ID/Acronym/Agency:
BRSG-05357/RS/DRS NIH HHS; CA-15823/CA/NCI NIH HHS; HL-31408/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
9001-25-6/Factor VII; 9001-29-0/Factor X; 9035-58-9/Thromboplastin; EC 3.4.21.21/Factor VIIa

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Consumptive coagulopathy, fibrinolysis and protease-antiprotease interactions during acute human pan...
Next Document:  Comparative study of antithrombin III.protease complex metabolism by fibroblasts and vascular endoth...