Document Detail


Tissue extract from dopamine-depleted striatum enhances differentiation of cultured striatal type-1 astrocytes.
MedLine Citation:
PMID:  8852591     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of tissue extract from dopamine (DA)-depleted striatum (lesion extract, L-ext) on morphological and electrophysiological natures of cultured striatal astrocytes were investigated. L-ext treatment suppressed the proliferation of type-1 astrocytes. They became fibrous in a concentration-dependent manner. These changes were not observed in type-2 astrocytes. By whole cell patch-clamp recording, two kinetically and pharmacologically distinct voltage-activated potassium currents, A current and delayed rectifier, were identified. L-ext treatment enhanced both currents in type-1 astrocytes, but only A current in type-2. Data suggest that in tissue extract from DA-depleted striatum, there are increased trophic activities that promote the differentiation of type-1 astrocytes.
Authors:
Y Shimano; A Fukuda; I Fujimoto; H Hida; K Nakajima; A Okabe; H Nishino
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neuroscience letters     Volume:  205     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1996-12-16     Completed Date:  1996-12-16     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  193-6     Citation Subset:  IM    
Affiliation:
Department of Physiology, Nagoya City University Medical School, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Astrocytes / physiology*,  ultrastructure
Cell Differentiation / physiology
Cell Division / physiology
Cell Extracts
Cells, Cultured / physiology,  ultrastructure
Dopamine / deficiency*
Immunohistochemistry
Neostriatum / cytology*,  physiology
Patch-Clamp Techniques
Potassium Channels / metabolism
Rats
Rats, Wistar
Sodium Channels / metabolism
Chemical
Reg. No./Substance:
0/Cell Extracts; 0/Potassium Channels; 0/Sodium Channels

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