Document Detail

Tissue engineering of lips and muco-cutaneous junctions: in vitro development of tissue engineered constructs of oral mucosa and skin for lip reconstruction.
MedLine Citation:
PMID:  22067042     Owner:  NLM     Status:  MEDLINE    
We report for the first time the fabrication of a three-dimensional tissue structure containing, in a continuous layer, the morphological features of a lip: epidermal skin, vermillion, and oral mucosa. This tissue engineered muco-cutaneous (M/C) equivalent was manufactured using human oral and skin keratinocytes grown on an acellular, nonimmunogenic dermal equivalent (AlloDerm(®)) to produce a tissue equivalent with similar anatomic and handling properties as native human lips. Confirmation of the structural composition of the construct was performed using routine histology and immunohistochemistry by identification of epithelial markers that are differentially expressed in separate anatomic areas of the lips. These full-thickness human lip skin equivalents can be used in surgical lip reconstruction in individuals suffering from lip loss from cancer, congenital deformations, and injuries after accidents. We propose this technique can be used as a general basis for tissue engineering of M/C junctions in other parts of the body, such as anus and vagina.
Antonio Peramo; Cynthia L Marcelo; Stephen E Feinberg
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-12-28
Journal Detail:
Title:  Tissue engineering. Part C, Methods     Volume:  18     ISSN:  1937-3392     ISO Abbreviation:  Tissue Eng Part C Methods     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-03-27     Completed Date:  2012-09-28     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  101466663     Medline TA:  Tissue Eng Part C Methods     Country:  United States    
Other Details:
Languages:  eng     Pagination:  273-82     Citation Subset:  IM    
Department of Oral and Maxillofacial Surgery, University of Michigan, Ann Arbor, MI 48109-5018, USA.
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MeSH Terms
Cell Movement
Cell Proliferation
Keratins / metabolism
Lip / physiology*
Mouth Mucosa / anatomy & histology*
Reconstructive Surgical Procedures / methods*
Skin / anatomy & histology*
Tissue Engineering / methods*
Tissue Scaffolds / chemistry*
Grant Support
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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