Document Detail


Tissue engineering of a collagen-based vascular media: Demonstration of functionality.
MedLine Citation:
PMID:  21220958     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The property of vasoactivity is important for both resistance vessels and larger arteries. Evaluation of smooth muscle cell phenotype is often done in place of functional testing in engineered tissues, assuming a direct correlation between cell phenotype and tissue contractile force. In this study we look at a large panel of vasoactive agents to determine the functionality of our collagen-based tissue. The engineered vascular media elicited a measurable change in force in response to seven of the nine agents used. As part of this characterization, TGF-β1 and TNF-α were used to promote a more contractile and synthetic cell phenotype respectively. Both smooth muscle α-actin and vasoconstriction were evaluated in ring sections. Due to large differences in cell-compaction and cell distribution in the tissues, no correlation was found between α-actin expression and contractile strength. This highlights the need for functional testing of engineered tissue and the importance of cell-matrix interactions in vasoactivity.
Authors:
Stacey C Schutte; Zhenzhen Chen; Kelvin G M Brockbank; Robert M Nerem
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Organogenesis     Volume:  6     ISSN:  1555-8592     ISO Abbreviation:  Organogenesis     Publication Date:    2010 Oct-Dec
Date Detail:
Created Date:  2011-01-11     Completed Date:  2011-06-06     Revised Date:  2013-07-02    
Medline Journal Info:
Nlm Unique ID:  101253266     Medline TA:  Organogenesis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  204-11     Citation Subset:  IM    
Affiliation:
Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aorta / cytology
Cell Culture Techniques
Collagen* / metabolism
Immunohistochemistry
Rats
Tissue Engineering*
Tunica Media / anatomy & histology,  cytology*,  drug effects
Vasodilator Agents / pharmacology
Chemical
Reg. No./Substance:
0/Vasodilator Agents; 9007-34-5/Collagen
Comments/Corrections

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