| Tissue engineering of a collagen-based vascular media: Demonstration of functionality. | |
| | |
MedLine Citation:
|
PMID: 21220958 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The property of vasoactivity is important for both resistance vessels and larger arteries. Evaluation of smooth muscle cell phenotype is often done in place of functional testing in engineered tissues, assuming a direct correlation between cell phenotype and tissue contractile force. In this study we look at a large panel of vasoactive agents to determine the functionality of our collagen-based tissue. The engineered vascular media elicited a measurable change in force in response to seven of the nine agents used. As part of this characterization, TGF-β1 and TNF-α were used to promote a more contractile and synthetic cell phenotype respectively. Both smooth muscle α-actin and vasoconstriction were evaluated in ring sections. Due to large differences in cell-compaction and cell distribution in the tissues, no correlation was found between α-actin expression and contractile strength. This highlights the need for functional testing of engineered tissue and the importance of cell-matrix interactions in vasoactivity. |
| | |
Authors:
|
Stacey C Schutte; Zhenzhen Chen; Kelvin G M Brockbank; Robert M Nerem |
Related Documents
:
|
16085008 - "gap guidance" of fibroblasts and epithelial cells by discontinuous edged surfaces. 3553868 - Cytopathogenic effect of salmonella typhi gifu 10007 on m cells of murine ileal peyer's... 17921358 - Conditional deletion of pten causes bronchiolar hyperplasia. 7368238 - Changes in the intestinal epithelial cells in abdominally irradiated germfree piglets. 21374648 - High-performance affinity purification for identification of 15-deoxy-Δ(12,14) -pgj(2)... 9441688 - Cell death in the avian sclerotome. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Organogenesis Volume: 6 ISSN: 1555-8592 ISO Abbreviation: Organogenesis Publication Date: 2010 Oct-Dec |
Date Detail:
|
Created Date: 2011-01-11 Completed Date: 2011-06-06 Revised Date: 2011-07-27 |
Medline Journal Info:
|
Nlm Unique ID: 101253266 Medline TA: Organogenesis Country: United States |
Other Details:
|
Languages: eng Pagination: 204-11 Citation Subset: IM |
Affiliation:
|
Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Aorta / cytology Cell Culture Techniques Collagen* / metabolism Immunohistochemistry Rats Tissue Engineering* Tunica Media / anatomy & histology, cytology*, drug effects Vasodilator Agents / pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Vasodilator Agents; 9007-34-5/Collagen |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Biodegradable microfluidic scaffolds for tissue engineering from amino alcohol-based poly(ester amid...
Next Document: Microdissection of the gene expression codes driving nephrogenesis.