Document Detail


Tissue-engineered arterial grafts: long-term results after implantation in a small animal model.
MedLine Citation:
PMID:  19524728     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Use of prosthetic vascular grafts in pediatric vascular surgical applications is limited because of risk of infection, poor durability, potential for thromboembolic complications, and lack of growth potential. Construction of an autologous neovessel using tissue engineering technology offers the potential to create an improved vascular conduit for use in pediatric vascular applications. METHODS: Tissue-engineered vascular grafts were assembled from biodegradable tubular scaffolds fabricated from poly-L-lactic acid mesh coated with epsilon-caprolactone and L-lactide copolymer. Thirteen scaffolds were seeded with human aortic endothelial and smooth muscle cells and implanted as infrarenal aortic interposition grafts in SCID/bg mice. Grafts were analyzed at time-points ranging from 4 days to 1 year after implantation. RESULTS: All grafts remained patent without evidence of thromboembolic complications, graft stenosis, or graft rupture as documented by serial ultrasound and computed tomographic angiogram, and confirmed histologically. All grafts demonstrated extensive remodeling leading to the development of well-circumscribed neovessels with an endothelial inner lining, neomedia containing smooth muscle cells and elastin, and a collagen-rich extracellular matrix. CONCLUSIONS: The development of second-generation tissue-engineered vascular grafts shows marked improvement over previous grafts and confirms feasibility of using tissue engineering technology to create an improved arterial conduit for use in pediatric vascular surgical applications.
Authors:
Tamar L Mirensky; Gregory N Nelson; Matthew P Brennan; Jason D Roh; Narutoshi Hibino; Tai Yi; Toshiharu Shinoka; Christopher K Breuer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of pediatric surgery     Volume:  44     ISSN:  1531-5037     ISO Abbreviation:  J. Pediatr. Surg.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-06-15     Completed Date:  2009-09-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0052631     Medline TA:  J Pediatr Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1127-32; discussion 1132-3     Citation Subset:  IM    
Affiliation:
Yale University School of Medicine, New Haven, CT 06510, USA. tamar.mirensky@yale.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Vessel Prosthesis*
Blood Vessel Prosthesis Implantation*
Cells, Cultured
Female
Humans
Mice
Mice, SCID
Models, Animal
Muscle, Smooth, Vascular
Myocytes, Smooth Muscle
Pilot Projects
Tissue Engineering*
Tissue Scaffolds

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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