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Tissue-engineered allograft intervertebral disc transplantation for the treatment of degenerative disc disease: experimental study in a beagle model.
MedLine Citation:
PMID:  22849557     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Objectives: To investigate whether the intervention of NP cells or hTERT gene transfected NP cells can prevent the degeneration process after allograft total disc transplantation. Methods: Eighteen canine lumbar intervertebral discs were obtained from 5 canines and cryopreserved in liquid nitrogen. Canine nucleus pulposus cells were isolated and transduced with AAV-hTERT. The cells were injected into the discs to construct a "tissue-engineered" allograft disc(group A). NP cells and DMEM/F12 were used for positive control (group B) and blank control(group C). 18 beagle dogs received the 3 groups of allograft IVD composites implantation respectively. Radiographic examination were performed at 4, 8 and 12 weeks post implantation. At 12 weeks after operation, all dogs were sacrificed and the lumbar spines were harvested for the biomechanical analysis then the allografts were undergone histological analysis, ectogenic NP cell tracing and hTERT mRNA analysis. Results: Bony fusion between intervertebral disc allograft and adjacent host intervertebral body were observed in all animal. The disc height and T2 signal intensity preservation in group A and B was better than group C. MRI showed typical degenerative changes in group C. In group A, the normalized grayscale of the transplanted disc on MRI image was significant higher than that of the controls at 12 weeks. Biomechanical test showed a poor stability preservation in group C compare to group A and B. PKH-26 positive cells were identified within the allograft discs in group A at 12 weeks, providing evidence of cell survival. Histological analysis showed the NP cell morphology, cell number and distribution of the allograft discs was better preserved in group A and B compared to group C at 12 weeks follow up. Conclusion: The present study demonstrated that NP cells or hTERT loaded NP cells intervention could effectively resist the degeneration of the allogenic transplanted intervertebral discs in a beagle model. The hTERT loaded NP cells had a better anti-degeneration effect on the transplanted disc than NP cells. This modified disc regeneration technique through NP cell injection or manipulation may have the potential to ensure the long term function preservation of allograft disc transplantation.
Authors:
Hongkui Xin; Chao Zhang; Deli Wang; Zhiyuan Shi; Tao Gu; Chaofeng Wang; Jianhong Wu; Yan Zhang; Qing He; Dike Ruan
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-31
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  -     ISSN:  1937-335X     ISO Abbreviation:  Tissue Eng Part A     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-8-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Xijing Hospital, The Fourth Military Medical University, Department of of Orthopedics and Traumatology, Xi'an, China; xintodorov@yahoo.com.cn.
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