Document Detail

Tissue distribution, synthesis stage, and ethylene induction of pineapple (Ananas comosus) chitinases.
MedLine Citation:
PMID:  15849431     Owner:  NLM     Status:  MEDLINE    
We examined the tissue distribution, synthesis stage, and ethylene induction of three types of pineapple chitinase using chitinase activity gel and immunoblot analysis. Type A (acidic class III) exists in all tissues, while type B (weakly basic class I, which has strong antifungal activity) and type C (acidic class I) are localized mainly in the leaf and stem. In a pericarp, type A exists at all stages during fruit development, while type B and type C exist only at the early stage. Synthesis of type A is induced by ethylene, while that of types B and C is not affected by it. These results suggest that the physiological roles of these three types of chitinase in pineapple are different.
Toki Taira; Noriko Toma; Marika Ichi; Makoto Takeuchi; Masanobu Ishihara
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  69     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-04-25     Completed Date:  2005-09-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  852-4     Citation Subset:  IM    
Department of Bioscience and Biotechnology, Faculty of Agriculture, Ryukyu University, Okinawa 903-0213, Japan.
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MeSH Terms
Ananas / drug effects*,  enzymology*,  growth & development
Chitinase / metabolism*
Enzyme Induction / drug effects
Ethylenes / pharmacology*
Fruit / drug effects,  enzymology,  growth & development
Gene Expression Profiling
Gene Expression Regulation, Plant
Reg. No./Substance:
0/Ethylenes; 74-85-1/ethylene; EC

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