| Tissue distribution and cardiac metabolism of 3-iodothyronamine. | |
| | |
MedLine Citation:
|
PMID: 20739399 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
3-iodothyronamine (T1AM) is a novel relative of thyroid hormone, able to interact with specific G protein-coupled receptors, known as trace amine-associated receptors. Significant functional effects are produced by exogenous T1AM, including a negative inotropic and chronotropic effect in cardiac preparations. This work was aimed at estimating endogenous T1AM concentration in different tissues and determining its cardiac metabolism. A novel HPLC tandem mass spectrometry assay was developed, allowing detection of T1AM, thyronamine, 3-iodothyroacetic acid, and thyroacetic acid. T1AM was detected in rat serum, at the concentration of 0.3±0.03 pmol/ml, and in all tested organs (heart, liver, kidney, skeletal muscle, stomach, lung, and brain), at concentrations significantly higher than the serum concentration, ranging from 5.6±1.5 pmol/g in lung to 92.9±28.5 pmol/g in liver. T1AM was also identified for the first time in human blood. In H9c2 cardiomyocytes and isolated perfused rat hearts, significant Na+-dependent uptake of exogenous T1AM was observed, and at the steady state total cellular or tissue T1AM concentration exceeded extracellular concentration by more than 20-fold. In both preparations T1AM underwent oxidative deamination to 3-iodothyroacetic acid. T1AM deamination was inhibited by iproniazid but not pargyline or semicarbazide, suggesting the involvement of both monoamine oxidase and semicarbazide-sensitive amine oxidase. Thyronamine and thyroacetic acid were not detected in heart. Finally, evidence of T1AM production was observed in cardiomyocytes exposed to exogenous thyroid hormone, although the activity of this pathway was very low. |
| | |
Authors:
|
Alessandro Saba; Grazia Chiellini; Sabina Frascarelli; Maja Marchini; Sandra Ghelardoni; Andrea Raffaelli; Massimo Tonacchera; Paolo Vitti; Thomas S Scanlan; Riccardo Zucchi |
Related Documents
:
|
19073199 - Role of 1alpha,25(oh)2 vitamin d3 on alpha-[1-(14)c]meaib accumulation in immature rat ... 6774799 - Stimulation of lh and fsh secretion following intraventicular injection of cysteic acid... 17199429 - Effects of tsh receptor mutations on binding and biological activity of monoclonal anti... 12093449 - Use of amino acids as growth hormone-releasing agents by athletes. 1257259 - Studies on the isolation and chemical properties of porcine follicle-stimulating hormone. 13943859 - Cysteine stimulation of glucose oxidation in thyroid tissue. 14706739 - Synthesis and properties of methyl 5-(1'r,2's)-(2-octadecylcycloprop-1-yl)pentanoate an... 6477599 - Odd-carbon fatty acid metabolism in hepatocytes from starved rats. 1874839 - High-performance liquid chromatographic separation of bile acids and bile alcohols dias... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-25 |
Journal Detail:
|
Title: Endocrinology Volume: 151 ISSN: 1945-7170 ISO Abbreviation: Endocrinology Publication Date: 2010 Oct |
Date Detail:
|
Created Date: 2010-09-22 Completed Date: 2010-11-04 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: United States |
Other Details:
|
Languages: eng Pagination: 5063-73 Citation Subset: AIM; IM |
Affiliation:
|
Dipartimento di Chimica e Chimica Industriale, University of Pisa, Via Roma 55, 56126 Pisa, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cells, Cultured Chromatography, High Pressure Liquid Humans Kidney / chemistry, metabolism Liver / chemistry, metabolism Lung / chemistry, metabolism Male Muscle, Skeletal / chemistry, metabolism Myocardium / chemistry, metabolism* Myocytes, Cardiac / chemistry, metabolism Rats Rats, Wistar Stomach / chemistry, metabolism Tandem Mass Spectrometry Thyronines / analysis, blood, metabolism* Tissue Distribution |
| Chemical | |
Reg. No./Substance:
|
0/3-iodothyronamine; 0/Thyronines |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: C1q/TNF-Related Protein-3 Represents a Novel and Endogenous Lipopolysaccharide Antagonist of the Adi...
Next Document: GADD45gamma: a new vitamin D-regulated gene that is antiproliferative in prostate cancer cells.