Document Detail


Tissue contribution to the mechanical features of diaphragmatic initial lymphatics.
MedLine Citation:
PMID:  20724369     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The role of the mechanical properties of the initial lymphatic wall and of the surrounding tissue in supporting lymph formation and/or progression was studied in six anaesthetized, neuromuscularly blocked and mechanically ventilated rats. After mid-sternal thoracotomy, submesothelial initial lymphatics were identified on the pleural diaphragmatic surface through stereomicroscopy. An 'in vivo' lymphatic segment was prepared by securing two surgical threads around the vessel at a distance of ∼2.5 mm leaving the vessel in place. Two glass micropipettes were inserted into the lumen, one for intraluminar injections of 4.6 nl saline boluses and one for hydraulic pressure (Plymph) recording. The compliance of the vessel wall (Clymph) was calculated as the slope of the plot describing the change in segment volume as a function of the post-injection Plymph changes. Two superficial lymphatic vessel populations with a significantly different Clymph (6.7 ± 1.6 and 1.5 ± 0.4 nl mmHg−1 (mean ± S.E.M.), P < 0.001) were identified. In seven additional rats, the average elastic modulus of diaphragmatic tissue strips was determined by uniaxial tension tests to be 1.7 ± 0.3 MPa. Clymph calculated for an initial lymphatic completely surrounded by isotropic tissue was 0.068 nl mmHg−1, i.e. two orders of magnitude lower than in submesothelial lymphatics. Modelling of stress distribution in the lymphatic wall suggests that compliant vessels may act as reservoirs accommodating large absorbed fluid volumes, while lymphatics with stiffer walls serve to propel fluid through the lumen of the lymphatic vessel by taking advantage of the more efficient mechanical transmission of tissue stresses to the lymphatic lumen.
Authors:
Andrea Moriondo; Federica Boschetti; Francesca Bianchin; Simone Lattanzio; Cristiana Marcozzi; Daniela Negrini
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of physiology     Volume:  588     ISSN:  1469-7793     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-22     Completed Date:  2011-01-28     Revised Date:  2013-05-28    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  3957-69     Citation Subset:  IM    
Affiliation:
Department of Experimental and Clinical Biomedical Sciences, Università degli Studi dell’Insubria, 21100 Varese, Italy.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Diaphragm / physiology*
Lymphatic System / physiology*
Male
Pleura / physiology
Rats
Rats, Wistar
Respiration, Artificial
Statistics, Nonparametric
Stress, Mechanical
Comments/Corrections

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