Document Detail


Tips and tricks for preparing lampbrush chromosome spreads from Xenopus tropicalis oocytes.
MedLine Citation:
PMID:  20085818     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Due to their large size and fine organization, lampbrush chromosomes (LBCs) of amphibian oocytes have been for decades one of the favorite tools of biologists for the analysis of transcriptional and post-transcriptional processes at the cytological level. The emergence of the diploid Xenopus tropicalis amphibian as a model organism for vertebrate developmental genetics and the accumulation of sequence data made available by its recent genomic sequencing, strongly revive the interest of LBCs as a powerful tool to study genes expressed during oogenesis. We describe here a detailed protocol for preparing LBCs from X. tropicalis oocyte and give practical advice to encourage a large number of researchers to become familiar with these chromosomes.
Authors:
May Penrad-Mobayed; Rasha Kanhoush; Caroline Perrin
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Publication Detail:
Type:  Journal Article     Date:  2010-01-18
Journal Detail:
Title:  Methods (San Diego, Calif.)     Volume:  51     ISSN:  1095-9130     ISO Abbreviation:  Methods     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-11     Completed Date:  2010-10-22     Revised Date:  2011-04-13    
Medline Journal Info:
Nlm Unique ID:  9426302     Medline TA:  Methods     Country:  United States    
Other Details:
Languages:  eng     Pagination:  37-44     Citation Subset:  IM    
Affiliation:
Institut Jacques Monod, UMR7592, CNRS and University Paris Diderot-Paris 7, Department of Molecular and Cellular Pathology, Paris, France. penrad@ijm.univ-paris-diderot.fr
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MeSH Terms
Descriptor/Qualifier:
Algorithms
Animals
Chromosomes / ultrastructure
Cytogenetic Analysis / instrumentation,  methods*
Cytogenetics / methods*
Diploidy
Female
Meiosis
Microscopy, Phase-Contrast / methods
Models, Genetic
Oocytes / metabolism*
RNA Processing, Post-Transcriptional
Sequence Analysis, DNA
Transcription, Genetic
Xenopus / metabolism*
Comments/Corrections
Erratum In:
Methods. 2011 Mar;53(3):326

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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