Document Detail


Tin-protoporphyrin prevents experimental superficial siderosis in rabbits.
MedLine Citation:
PMID:  12152784     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Superficial siderosis of the human central nervous system is caused by small continuous or recurrent subarachnoid hemorrhages that lead to the destructive deposition of hemosiderin. The excessive tissue iron derives from heme that is oxidized in a rate-limiting step by the enzyme heme oxygenase (HO), and especially the inducible form, HO-1. We postulated that competitive inhibition of HO by tin-protoporphyrin IX (SnPP) could prevent experimental superficial siderosis. Since synthetic metalloporphyrins do not cross the blood-brain barrier, SnPP was delivered directly into the cisterna magna. Rabbits received weekly intracisternal injections of washed autologous red blood cells (RBC) over a period of 1 to 16 wk. In companion experiments, SnPP was added to the suspension of RBC, or SnPP was injected without RBC. All injections caused increased HO-1 immunoreactivity in the Bergmann glia of the cerebellar cortex and in superficial astrocytes of the piriform cortex. The injections of RBC or RBC with added SnPP also generated a vigorous microglial response. The metalloporphyrin entered the tissue in inhibitory amounts and greatly reduced the accumulation of histochemically detectable iron. It did not alter the microglial response. The observations allowed the conclusion that SnPP suppressed heme oxidation but did not affect other steps in the pathogenesis of superficial siderosis.
Authors:
Arnulf H Koeppen; Andrew C Dickson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of neuropathology and experimental neurology     Volume:  61     ISSN:  0022-3069     ISO Abbreviation:  J. Neuropathol. Exp. Neurol.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-08-02     Completed Date:  2002-08-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985192R     Medline TA:  J Neuropathol Exp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  689-701     Citation Subset:  IM    
Affiliation:
Neurology Service, VA Medical Center, Albany, New York 12208, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / metabolism
Cerebellar Cortex / enzymology
Enzyme Inhibitors / pharmacokinetics,  pharmacology*
Ferritins / metabolism
Heme Oxygenase (Decyclizing) / metabolism
Iron / metabolism
Male
Metalloporphyrins / pharmacokinetics,  pharmacology*
Protoporphyrins / pharmacokinetics,  pharmacology*
Rabbits
Siderosis / enzymology,  prevention & control*
Tissue Distribution
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Metalloporphyrins; 0/Protoporphyrins; 14325-05-4/tin protoporphyrin IX; 7439-89-6/Iron; 9007-73-2/Ferritins; EC 1.14.99.3/Heme Oxygenase (Decyclizing)

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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