Document Detail

Timing of zona pellucida formation in the tammar wallaby (Macropus eugenii) and brushtail possum (Trichosurus vulpecula).
MedLine Citation:
PMID:  9835379     Owner:  NLM     Status:  MEDLINE    
The zona pellucida (ZP) is an extracellular coat that surrounds the mammalian egg, and serves as the primary recognition site for fertilizing spermatozoa. The timetable of ZP formation was examined in two marsupials, the tammar wallaby (Macropus eugenii) and the brushtail possum (Trichosurus vulpecula) using conventional histological methods, immunofluorescence and electron microscopy. Ovaries from tammar wallaby pouch young less than 80 days of age contained only primordial follicles with a single layer of flattened granulosa cells. There was no evidence of ZP formation until 98 days, when a small number of eggs surrounded by a single layer of cuboidal granulosa cells had a ZP detectable by periodic-acid-schiff staining and rabbit anti-pig ZP polyclonal antibody labelling. Possum ovaries at 108 and 114 days also contained a small number of eggs with a ZP and a single layer of cuboidal granulosa cells. The antibody also labelled the peripheral cytoplasm of oocytes at this stage and, occasionally, the granulosa cells. Antral follicles were first detected at 144 days in the wallaby and 125 days in the possum, and always contained an egg surrounded by a ZP. Ovaries from 147, 158, 165, 181, 184 and 210-day-old tammar wallabies contained a range of follicle types from primordial through early antrum formation. Electron microscopy confirmed observations made at the light microscope level. The ZP was first detectable in small primary follicles with a single layer of cuboidal granulosa cells in areas where microvilli had begun to form on the egg plasma membrane. Immunogold labelling indicated the egg cytoplasm as the origin of the ZP proteins. The ZP completely filled the space between the egg and the adjacent granulosa cells in preantral follicles, so that there was no perivitelline space.
K E Mate
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Animal reproduction science     Volume:  53     ISSN:  0378-4320     ISO Abbreviation:  Anim. Reprod. Sci.     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1999-01-28     Completed Date:  1999-01-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7807205     Medline TA:  Anim Reprod Sci     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  237-52     Citation Subset:  IM    
Cooperative Research Centre for Conservation and Management of Marsupials, School of Biological Sciences, Macquarie University, New South Wales, Australia.
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MeSH Terms
Granulosa Cells / ultrastructure
Macropodidae / physiology*
Microscopy, Electron
Opossums / physiology*
Ovarian Follicle / physiology
Ovum / physiology,  ultrastructure
Periodic Acid-Schiff Reaction
Zona Pellucida / physiology*,  ultrastructure

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