Document Detail


Timing of estrogen replacement influences atherosclerosis progression and plaque leukocyte populations in ApoE-/- mice.
MedLine Citation:
PMID:  18374339     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Studies of the effects of estrogen replacement therapy on coronary heart disease risk have produced conflicting results. We hypothesize that this may be explained by differences in the length of estrogen deficiency prior to initiation of treatment and associated variation in plaque inflammation or stage of progression. The goal of this study was to determine whether estrogen administered after a period of deficiency affects plaque progression and leukocyte populations. Ovariectomized ApoE-/- mice were treated as follows: group 1: continuous estrogen for 90 days (E+/+); group 2: placebo for 45 days followed by estrogen for 45 days (E-/+); group 3: estrogen for 45 days followed by placebo for 45 days (E+/-); and group 4: placebo for 90 days (E-/-). Serum lipoprotein concentrations, plaque size and inflammatory cell (macrophage, CD3+, CD4+, CD8+, dendritic cell, and NK cell) densities were quantified. Plaque size was smaller in groups receiving early estrogen therapy. CD3+ and total inflammatory cell densities were lower in late estrogen therapy groups. The CD8 to dendritic cell ratio was significantly lower in the E-/+ group only. These results suggest that a period of estrogen deficiency followed by reintroduction alters the immunologic environment of atherosclerotic lesions as well as plaque progression.
Authors:
Jennifer A Cann; Thomas C Register; Michael R Adams; Richard W St Clair; Mark A Espeland; J Koudy Williams
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2008-02-21
Journal Detail:
Title:  Atherosclerosis     Volume:  201     ISSN:  1879-1484     ISO Abbreviation:  Atherosclerosis     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2009-10-30     Completed Date:  2010-01-26     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  0242543     Medline TA:  Atherosclerosis     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  43-52     Citation Subset:  IM    
Affiliation:
Department of Pathology, Comparative Medicine Clinical Research Center, Wake Forest University Health Sciences, Winston-Salem, NC 27157, United States. jcann@wfubmc.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Apolipoproteins E / physiology*
Atherosclerosis / blood*,  etiology,  pathology*
Drug Administration Schedule
Estradiol / administration & dosage*
Estrogen Replacement Therapy*
Estrogens / administration & dosage*
Female
Leukocyte Count
Mice
Mice, Inbred C57BL
Ovariectomy
Grant Support
ID/Acronym/Agency:
R01 AG017864-05/AG/NIA NIH HHS; R01AG17864/AG/NIA NIH HHS; T32 RR 07009/RR/NCRR NIH HHS; T32 RR007009-22/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Apolipoproteins E; 0/Estrogens; 50-28-2/Estradiol
Comments/Corrections

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