Document Detail

Timed feeding of mice modulates light-entrained circadian rhythms of reticulated platelet abundance and plasma thrombopoietin and affects gene expression in megakaryocytes.
MedLine Citation:
PMID:  19438469     Owner:  NLM     Status:  MEDLINE    
Circadian (c. 24 h) rhythms of physiology are entrained to either the environmental light-dark cycle or the timing of food intake. In the current work the hypothesis that rhythms of platelet turnover in mammals are circadian and entrained by food intake was explored in mice. Mice were entrained to 12 h light-dark cycles and given either ad libitum (AL) or restricted access (RF) to food during the light phase. Blood and megakaryocytes were then collected from mice every 4 h for 24 h. It was found that total and reticulated platelet numbers, plasma thrombopoietin (TPO) concentration and the mean size of mature megakaryocytes were circadian but not entrained by food intake. In contrast, a circadian rhythm in the expression of Arnt1 in megakaryocytes was entrained by food. Although not circadian, the expression in megakaryocytes of Nfe2, Gata1, Itga2b and Tubb1 expression was downregulated by RF, whereas Ccnd1 was not significantly affected by the feeding protocol. It is concluded that circadian rhythms of total platelet number, reticulated platelet number and plasma TPO concentration are entrained by the light-dark cycle rather than the timing of food intake. These findings imply that circadian clock gene expression regulates platelet turnover in mammals.
Paul S Hartley; John Sheward; Emma Scholefield; Karen French; Jacqueline M Horn; Megan C Holmes; Anthony J Harmar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-04
Journal Detail:
Title:  British journal of haematology     Volume:  146     ISSN:  1365-2141     ISO Abbreviation:  Br. J. Haematol.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-07-14     Completed Date:  2009-11-04     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  0372544     Medline TA:  Br J Haematol     Country:  England    
Other Details:
Languages:  eng     Pagination:  185-92     Citation Subset:  IM    
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MeSH Terms
Analysis of Variance
Blood Platelets / physiology*
Carrier Proteins / metabolism
Circadian Rhythm / physiology*
Cyclin D1 / metabolism
Feeding Behavior / physiology*
Fetal Proteins / metabolism
Gene Expression
Megakaryocytes / physiology*
Mice, Inbred C57BL
Microtubule-Associated Proteins
Photic Stimulation*
Platelet Count
Thrombopoiesis / physiology
Thrombopoietin / metabolism*
Time Factors
Transcription Factors / metabolism
Tubulin / metabolism
Grant Support
G9719726//Medical Research Council; //Medical Research Council
Reg. No./Substance:
0/Carrier Proteins; 0/Ccnd1 protein, mouse; 0/Fetal Proteins; 0/Microtubule-Associated Proteins; 0/TACC3 protein, mouse; 0/Transcription Factors; 0/Tubulin; 136601-57-5/Cyclin D1; 9014-42-0/Thrombopoietin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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