| Time of sampling is crucial for measurement of cell-free plasma DNA following acute aseptic inflammation induced by exercise. | |
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MedLine Citation:
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PMID: 20800058 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: To determine the time-course changes of cell-free plasma DNA (cfDNA) following heavy exercise. METHODS: cfDNA concentration, C-reactive protein levels (hs-CRP), uric acid concentration (UA), creatine kinase activity (CK) were measured before and post-exercise (immediately post, 0.5h, 1h, 2h, 3h, 4h, 5h, 6h, 8h, 10h, 24h). RESULTS: cfDNA increased (15-fold) 30-min post-exercise and normalized thereafter. hs-CRP increased (56%, p<0.001) 1h post-exercise, remained elevated throughout recovery (52-142%, p<0.0001), and peaked (200% rise, p<0.0001) at 24h post-exercise. UA and CK increased (p<0.05), immediately post-exercise, remained elevated throughout recovery (p<0.0001), and peaked (p<0.0001) at 24h of post-exercise recovery. CONCLUSIONS: cfDNA sampling timing is crucial and a potential source of error following aseptic inflammation. |
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Authors:
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Ioannis G Fatouros; Athanasios Z Jamurtas; Michalis G Nikolaidis; Aspasia Destouni; Yiannis Michailidis; Christina Vrettou; Ioannis I Douroudos; Alexandra Avloniti; Athanasios Chatzinikolaou; Kiriakos Taxildaris; Emmanouel Kanavakis; Ioannis Papassotiriou; Dimitrios Kouretas |
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Publication Detail:
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Type: Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-25 |
Journal Detail:
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Title: Clinical biochemistry Volume: 43 ISSN: 1873-2933 ISO Abbreviation: Clin. Biochem. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-11 Completed Date: 2011-01-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0133660 Medline TA: Clin Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 1368-70 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Physical Education and Sports Science, Democritus University of Thrace, Komotini 69100, Greece. ifatouro@phyed.duth.gr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acute Disease Asepsis* C-Reactive Protein / metabolism Cell-Free System Creatine Kinase / blood DNA / blood* Exercise / physiology* Humans Inflammation / blood*, physiopathology* Male Rest / physiology Specimen Handling / methods* Time Factors Uric Acid / blood Young Adult |
| Chemical | |
Reg. No./Substance:
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69-93-2/Uric Acid; 9007-41-4/C-Reactive Protein; 9007-49-2/DNA; EC 2.7.3.2/Creatine Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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