Document Detail

Time of sampling is crucial for measurement of cell-free plasma DNA following acute aseptic inflammation induced by exercise.
MedLine Citation:
PMID:  20800058     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: To determine the time-course changes of cell-free plasma DNA (cfDNA) following heavy exercise.
METHODS: cfDNA concentration, C-reactive protein levels (hs-CRP), uric acid concentration (UA), creatine kinase activity (CK) were measured before and post-exercise (immediately post, 0.5h, 1h, 2h, 3h, 4h, 5h, 6h, 8h, 10h, 24h).
RESULTS: cfDNA increased (15-fold) 30-min post-exercise and normalized thereafter. hs-CRP increased (56%, p<0.001) 1h post-exercise, remained elevated throughout recovery (52-142%, p<0.0001), and peaked (200% rise, p<0.0001) at 24h post-exercise. UA and CK increased (p<0.05), immediately post-exercise, remained elevated throughout recovery (p<0.0001), and peaked (p<0.0001) at 24h of post-exercise recovery.
CONCLUSIONS: cfDNA sampling timing is crucial and a potential source of error following aseptic inflammation.
Ioannis G Fatouros; Athanasios Z Jamurtas; Michalis G Nikolaidis; Aspasia Destouni; Yiannis Michailidis; Christina Vrettou; Ioannis I Douroudos; Alexandra Avloniti; Athanasios Chatzinikolaou; Kiriakos Taxildaris; Emmanouel Kanavakis; Ioannis Papassotiriou; Dimitrios Kouretas
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-25
Journal Detail:
Title:  Clinical biochemistry     Volume:  43     ISSN:  1873-2933     ISO Abbreviation:  Clin. Biochem.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-11     Completed Date:  2011-01-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0133660     Medline TA:  Clin Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1368-70     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
Department of Physical Education and Sports Science, Democritus University of Thrace, Komotini 69100, Greece.
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MeSH Terms
Acute Disease
C-Reactive Protein / metabolism
Cell-Free System
Creatine Kinase / blood
DNA / blood*
Exercise / physiology*
Inflammation / blood*,  physiopathology*
Rest / physiology
Specimen Handling / methods*
Time Factors
Uric Acid / blood
Young Adult
Reg. No./Substance:
69-93-2/Uric Acid; 9007-41-4/C-Reactive Protein; 9007-49-2/DNA; EC Kinase

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