Document Detail


Time-resolved measurements of intracellular ATP in the yeast Saccharomyces cerevisiae using a new type of nanobiosensor.
MedLine Citation:
PMID:  20880841     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adenosine 5'-triphosphate is a universal molecule in all living cells, where it functions in bioenergetics and cell signaling. To understand how the concentration of ATP is regulated by cell metabolism and in turn how it regulates the activities of enzymes in the cell it would be beneficial if we could measure ATP concentration in the intact cell in real time. Using a novel aptamer-based ATP nanosensor, which can readily monitor intracellular ATP in eukaryotic cells with a time resolution of seconds, we have performed the first on-line measurements of the intracellular concentration of ATP in the yeast Saccharomyces cerevisiae. These ATP measurements show that the ATP concentration in the yeast cell is not stationary. In addition to an oscillating ATP concentration, we also observe that the concentration is high in the starved cells and starts to decrease when glycolysis is induced. The decrease in ATP concentration is shown to be caused by the activity of membrane-bound ATPases such as the mitochondrial F(0)F(1) ATPase-hydrolyzing ATP and the plasma membrane ATPase (PMA1). The activity of these two ATPases are under strict control by the glucose concentration in the cell. Finally, the measurements of intracellular ATP suggest that 2-deoxyglucose (2-DG) may have more complex function than just a catabolic block. Surprisingly, addition of 2-DG induces only a moderate decline in ATP. Furthermore, our results suggest that 2-DG may inhibit the activation of PMA1 after addition of glucose.
Authors:
Veli C Ozalp; Tina R Pedersen; Lise J Nielsen; Lars F Olsen
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-29
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  285     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-24     Completed Date:  2010-12-30     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  37579-88     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK5230 Odense M, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphatases / metabolism
Adenosine Triphosphate / analysis*,  metabolism
Biosensing Techniques / methods*
Glycolysis
Intracellular Space / chemistry*,  metabolism
Nanotechnology / methods*
Saccharomyces cerevisiae / chemistry*,  enzymology,  metabolism
Saccharomyces cerevisiae Proteins / metabolism
Chemical
Reg. No./Substance:
0/Saccharomyces cerevisiae Proteins; 56-65-5/Adenosine Triphosphate; EC 3.6.1.-/Adenosine Triphosphatases
Comments/Corrections

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