Document Detail

Time-resolved immunofluorometric assay of pregnancy-associated plasma protein A in maternal serum screening for Down's syndrome in first trimester of pregnancy.
MedLine Citation:
PMID:  8896900     Owner:  NLM     Status:  MEDLINE    
A low maternal serum concentration of pregnancy associated plasma protein-A (MS-PAPP-A) in the first trimester has been suggested as a marker for the presence of a Down's syndrome (DS) fetus. We developed a time-resolved immunofluorometric assay (TrIFMA) for PAPP-A with a sensitivity < 3.9 mIU/l. In the 7-12 gestational weeks interval the median multiples of the median (MoM) was 0.57 (95%-confidence interval; 0.47-0.99) in DS pregnancies (n = 29) and lower than in controls (n = 223) (P < 0.005). The efficiency of MS-PAPP-A alone was evaluated using empirical receiver-operator-characteristics (ROC) and a sensitivity of about 25% was found for a false-positive rate of about 10% in the 7-12 gestational weeks interval. In parameterized ROC analysis a sensitivity of 9% was found for a false-positive rate of 5%. The TrIFMA PAPP-A assay seems to fulfil the quality criteria for an assay to be used in large-scale serum screening for Down's syndrome.
Q P Qin; T H Nguyen; M Christiansen; S O Larsen; B Nørgaard-Pedersen
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  254     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1997-02-07     Completed Date:  1997-02-07     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  113-29     Citation Subset:  IM    
Department of Clinical Biochemistry, Statens Seruminstitut, Copenhagen, Denmark.
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MeSH Terms
Down Syndrome / blood,  diagnosis*
Enzyme-Linked Immunosorbent Assay
Fluorescent Antibody Technique
Pregnancy Trimester, First
Pregnancy-Associated Plasma Protein-A / analysis*
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
Reg. No./Substance:
EC 3.4.24.-/Pregnancy-Associated Plasma Protein-A

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