| Time-resolved fluorescence spectroscopy as a diagnostic technique of oral carcinoma: Validation in the hamster buccal pouch model. | |
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MedLine Citation:
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PMID: 20157056 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To investigate the benefit of using time-resolved, laser-induced fluorescence spectroscopy for diagnosing malignant and premalignant lesions of the oral cavity. DESIGN: The carcinogen 7,12-dimethylbenz[a]anthracene (DMBA) was applied to 1 cheek pouch of 19 hamsters. The contralateral pouch and the cheek pouches of 3 hamsters without DMBA exposure served as controls. SETTING: University of California, Davis. PARTICIPANTS: Twenty-two golden/Syrian hamsters. INTERVENTION: A nitrogen pulse laser was used to induce tissue autofluorescence between the wavelengths of 360 and 650 nm. MAIN OUTCOME MEASURES: Spectral intensities and time-domain measurements were obtained and compared with the histopathologic findings at each corresponding site. RESULTS: Spectral intensities and lifetime values at 3 spectral bands (SBs; SB1 = 380 +/- 10 nm; SB2 = 460 +/- 10 nm, and SB3 = 635 +/- 10 nm) allowed for discrimination among healthy epithelium, dysplasia, carcinoma in situ, and invasive carcinoma. The lifetime values at SB2 were the most important when distinguishing the lesions using only time-resolved parameters. An algorithm combining spectral fluorescence parameters derived from both spectral and time-domain parameters (peak intensities, average fluorescence lifetimes, and the Laguerre coefficient [zero-order]) for healthy epithelium, dysplasia, carcinoma in situ, and invasive carcinoma provided the best diagnostic discrimination, with 100%, 100%, 69.2%, and 76.5% sensitivity and 100%, 92.2%, 97.1%, and 96.2% specificity, respectively. CONCLUSIONS: The addition of time-resolved fluorescence-derived parameters significantly improves the capability of fluorescence spectroscopy-based diagnostics in the hamster buccal pouch. This technique provides a potential noninvasive diagnostic instrument for head and neck cancer. |
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Authors:
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D Gregory Farwell; Jeremy D Meier; Jesung Park; Yang Sun; Heather Coffman; Brian Poirier; Jennifer Phipps; Steve Tinling; Danny J Enepekides; Laura Marcu |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Archives of otolaryngology--head & neck surgery Volume: 136 ISSN: 1538-361X ISO Abbreviation: Arch. Otolaryngol. Head Neck Surg. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-16 Completed Date: 2010-03-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8603209 Medline TA: Arch Otolaryngol Head Neck Surg Country: United States |
Other Details:
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Languages: eng Pagination: 126-33 Citation Subset: AIM; IM |
Affiliation:
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Department of Otolaryngology-Head and Neck Surgery, University of California, Davis Medical Center, Sacramento, CA 95817, USA. gregory.farwell@ucdmc.ucdavis.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Algorithms Animals Carcinoma, Squamous Cell / chemically induced, diagnosis* Cheek / pathology Cricetinae Disease Models, Animal Male Mesocricetus Mouth Neoplasms / chemically induced, diagnosis* Precancerous Conditions / chemically induced, diagnosis* Spectrometry, Fluorescence* |
| Grant Support | |
ID/Acronym/Agency:
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R01-HL67377/HL/NHLBI NIH HHS; UL1 RR024146/RR/NCRR NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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