Document Detail


Time-regulated drug delivery system based on coaxially incorporated platelet α-granules for biomedical use.
MedLine Citation:
PMID:  23198681     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: Platelet derivatives serve as an efficient source of natural growth factors. In the current study, α-granules were incorporated into coaxial nanofibers.
MATERIALS & METHODS: A nanofiber scaffold containing α-granules was prepared by coaxial electrospinning. The biological potential of the nanofiber scaffold was evaluated in chondrocyte and mesenchymal stem cell cultivation studies. Additionally, the concentration of TGF-β1 was determined.
RESULTS: Microscopy studies showed that intact α-granules were incorporated into the coaxial nanofibers. The cultivation tests showed that the novel scaffold stimulated viability and extracellular matrix production of chondrocytes and mesenchymal stem cells. In addition, the concentration of growth factors necessary for the induction of cell proliferation significantly decreased.
CONCLUSION: The system preserved α-granule bioactivity and stimulated cell viability and chondrogenic differentiation of mesenchymal stem cells. Core/shell nanofibers incorporating α-granules are a promising system for tissue engineering, particularly cartilage engineering.
Authors:
Matej Buzgo; Radka Jakubova; Andrea Mickova; Michala Rampichova; Eva Prosecka; Petra Kochova; David Lukas; Evžen Amler
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-12-02
Journal Detail:
Title:  Nanomedicine (London, England)     Volume:  8     ISSN:  1748-6963     ISO Abbreviation:  Nanomedicine (Lond)     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-07-10     Completed Date:  2014-02-07     Revised Date:  2014-07-31    
Medline Journal Info:
Nlm Unique ID:  101278111     Medline TA:  Nanomedicine (Lond)     Country:  England    
Other Details:
Languages:  eng     Pagination:  1137-54     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Survival / drug effects
Cells, Cultured
Chondrocytes / cytology,  drug effects
Cytoplasmic Granules / chemistry*
Drug Delivery Systems / methods*
Humans
Mesenchymal Stromal Cells / cytology,  drug effects
Microscopy, Confocal
Nanofibers / chemistry*
Tissue Scaffolds / chemistry
Transforming Growth Factor beta1
Chemical
Reg. No./Substance:
0/Transforming Growth Factor beta1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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