Document Detail

Time-profiling fluorescent reporters in the Arabidopsis root.
MedLine Citation:
PMID:  24306858     Owner:  NLM     Status:  MEDLINE    
Confocal laser scanning microscopy is a useful nondestructive approach for the visualization of fluorescent reporters in planta. Samples are usually placed between a slide and a cover slip which, although suited to single time-point imaging, does not allow long-term observation. Here, we describe a technique to monitor changes in fluorescence in the Arabidopsis root over a long period of time. Treatment can easily be performed, and this approach is suitable for use in low-throughput chemical screens. We also present a rapid method to analyze fluorescence intensity profiles generated using this protocol.
Antoine P Larrieu; Andrew P French; Tony P Pridmore; Malcolm J Bennett; Darren M Wells
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  1056     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2014  
Date Detail:
Created Date:  2013-12-05     Completed Date:  2014-07-16     Revised Date:  2014-11-05    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  11-7     Citation Subset:  IM    
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MeSH Terms
Arabidopsis / drug effects,  metabolism*
Culture Techniques
Drug Evaluation, Preclinical / methods
Microscopy, Confocal
Microscopy, Fluorescence
Plant Growth Regulators / pharmacology
Plant Roots / drug effects,  metabolism*
Seedling / drug effects,  metabolism*
Grant Support
//Biotechnology and Biological Sciences Research Council
Reg. No./Substance:
0/Plant Growth Regulators

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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