Document Detail


Time-profiling fluorescent reporters in the Arabidopsis root.
MedLine Citation:
PMID:  24306858     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Confocal laser scanning microscopy is a useful nondestructive approach for the visualization of fluorescent reporters in planta. Samples are usually placed between a slide and a cover slip which, although suited to single time-point imaging, does not allow long-term observation. Here, we describe a technique to monitor changes in fluorescence in the Arabidopsis root over a long period of time. Treatment can easily be performed, and this approach is suitable for use in low-throughput chemical screens. We also present a rapid method to analyze fluorescence intensity profiles generated using this protocol.
Authors:
Antoine P Larrieu; Andrew P French; Tony P Pridmore; Malcolm J Bennett; Darren M Wells
Related Documents :
23874608 - Is beauty in the face of the beholder?
23157078 - Improvement in the clinical practicability of roentgen stereophotogrammetric analysis (...
20061108 - Diet and gastrointestinal signal on t1-weighted magnetic resonance imaging of mice.
23975388 - A rapid automatic processing platform for bead label-assisted microarray analysis: appl...
8992208 - Frequency resolved single-shot mr imaging using stochastic k-space trajectories.
25173418 - Imaging proteomics for diagnosis, monitoring and prediction of alzheimer's disease.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  1056     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2014  
Date Detail:
Created Date:  2013-12-05     Completed Date:  2014-07-16     Revised Date:  2014-10-14    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  11-7     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Arabidopsis / drug effects,  metabolism*
Culture Techniques
Drug Evaluation, Preclinical / methods
Microscopy, Confocal
Microscopy, Fluorescence
Plant Growth Regulators / pharmacology
Plant Roots / drug effects,  metabolism*
Seedling / drug effects,  metabolism*
Grant Support
ID/Acronym/Agency:
BB/G023972/1//Biotechnology and Biological Sciences Research Council; //Biotechnology and Biological Sciences Research Council
Chemical
Reg. No./Substance:
0/Plant Growth Regulators

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Fully Automated Compound Screening in Arabidopsis thaliana Seedlings.
Next Document:  Screening for Bioactive Small Molecules by In Vivo Monitoring of Luciferase-Based Reporter Gene Expr...