| Time-lapse observation of cellular function with fluorescent probe reveals novel CTL-target cell interactions. | |
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MedLine Citation:
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PMID: 19710208 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fluorescent protein that detects caspase-3 activation was used for the time-lapse observation of CTL-target cell interaction. In the target cells transfected with SCAT3.1 (caspase-3-sensitive fusion protein) complementary DNA, caspase-3 activation can be detected significantly earlier than the commonly used annexin-V binding that detects membrane change in apoptotic cells. Moreover, during the cytolytic interaction between OE4 CTL and W3 tumor target cells, detachment of CTL from the target cells occurred prior to caspase-3 activation and death of the target cells, indicating very early sensing of apoptotic target cells by CTL. This early detachment of OE4 CTL from W3 target cells was inhibited by the expression of CD80 co-stimulatory molecule on the target cells. Taken together, time-lapse observation of cellular interaction with functional probe, SCAT3.1 provides new kinetic information and demonstrates that co-stimulatory molecules regulate the kinetics of CTL-target cell interaction. |
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Authors:
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Michio Tomura; Yoshiko Saito Mori; Ryousuke Watanabe; Masato Tanaka; Atsushi Miyawaki; Osami Kanagawa |
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Publication Detail:
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Type: Journal Article Date: 2009-08-26 |
Journal Detail:
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Title: International immunology Volume: 21 ISSN: 1460-2377 ISO Abbreviation: Int. Immunol. Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-09-25 Completed Date: 2009-12-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8916182 Medline TA: Int Immunol Country: England |
Other Details:
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Languages: eng Pagination: 1145-50 Citation Subset: IM |
Affiliation:
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Research Center for Allergy and Immunology, RIKEN, Yokohama Institute, Tsurumi, Yokohama City, Kanagawa, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD80
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immunology,
metabolism Antigens, CD86 / immunology, metabolism Apoptosis / drug effects, immunology* Caspase 3 / drug effects, immunology*, metabolism Cell Line Cell Line, Tumor Fas Ligand Protein / pharmacology Fluorescent Dyes / metabolism Humans Macrophages / drug effects, immunology*, metabolism Microscopy, Confocal / methods Phagocytosis / drug effects, immunology Recombinant Fusion Proteins / immunology*, metabolism T-Lymphocytes, Cytotoxic / immunology*, metabolism Transfection |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD80; 0/Antigens, CD86; 0/Fas Ligand Protein; 0/Fluorescent Dyes; 0/Recombinant Fusion Proteins; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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