Document Detail

Time-lapse observation of cell alignment on nanogrooved patterns.
MedLine Citation:
PMID:  19324685     Owner:  NLM     Status:  MEDLINE    
Cells elongate on a surface with nanogrooved (NG) patterns and align along that pattern. Although various models have been proposed for how this occurs, much remains to be clarified. Studies with fixed cells do not lend themselves to answering some of these open questions. In this study, the dynamic behaviours of living mesenchymal stem cells on an NG substrate with a 200 nm groove depth, an 870 nm ridge width and a 670 nm groove width were observed using time-lapse microscopes. We found that filopodia moved as if they were probing the surroundings of the cell protrusion, and then some cell protrusions invaded the probed areas. Cell protrusions that extended perpendicular to the NG direction tended to retract more rapidly than those parallel to the grooves. From these facts, we think that the retracting phase of cell protrusions play a rule in cell alignment along the NG patterns.
Satoshi Fujita; Masahiro Ohshima; Hiroo Iwata
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-02-25
Journal Detail:
Title:  Journal of the Royal Society, Interface / the Royal Society     Volume:  6 Suppl 3     ISSN:  1742-5662     ISO Abbreviation:  J R Soc Interface     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-04-27     Completed Date:  2009-08-14     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  101217269     Medline TA:  J R Soc Interface     Country:  England    
Other Details:
Languages:  eng     Pagination:  S269-77     Citation Subset:  IM    
Department of Reparative Materials, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
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MeSH Terms
Cell Adhesion
Cell Culture Techniques*
Cell Enlargement
Cell Movement
Mesenchymal Stromal Cells / cytology*,  ultrastructure
Pseudopodia / physiology,  ultrastructure
Surface Properties

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