Document Detail


Time-lapse confocal imaging of development of Bacillus anthracis in macrophages.
MedLine Citation:
PMID:  15031802     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Macrophages attempt to battle infection with Bacillus anthracis spores by phagocytosis of the spores. However, it is believed that B. anthracis spores may survive phagocytosis and may actually use the macrophages that ingest them as a means of transport to lymph nodes. Thus far, the events that occur after spores undergo phagocytosis have remained unclear. To elucidate the fate of spores internalized by macrophages, we have used time-lapse confocal microscopy to follow individual fluorescent spores over time. By use of this method, we have determined that some phagocytized spores survive beyond germination, to become bacilli that then replicate within the macrophages.
Authors:
Gordon Ruthel; Wilson J Ribot; Sina Bavari; Timothy A Hoover
Publication Detail:
Type:  Journal Article     Date:  2004-03-19
Journal Detail:
Title:  The Journal of infectious diseases     Volume:  189     ISSN:  0022-1899     ISO Abbreviation:  J. Infect. Dis.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-03-19     Completed Date:  2004-05-13     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0413675     Medline TA:  J Infect Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1313-6     Citation Subset:  AIM; IM    
Affiliation:
Clinical Research Management, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland 21702, USA. gordon.ruthel@amedd.army.mil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacillus anthracis / growth & development,  immunology,  physiology*
Carbocyanines / chemistry
Fluorescent Dyes / chemistry
Macrophages / immunology,  microbiology*
Mice
Microscopy, Confocal
Organic Chemicals
Phagocytosis / physiology
Spores, Bacterial
Chemical
Reg. No./Substance:
0/3,3'-dihexadecylindocarbocyanine; 0/Carbocyanines; 0/Fluorescent Dyes; 0/Organic Chemicals; 0/SYTOX Green

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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