| Time-lapse confocal imaging of development of Bacillus anthracis in macrophages. | |
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MedLine Citation:
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PMID: 15031802 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Macrophages attempt to battle infection with Bacillus anthracis spores by phagocytosis of the spores. However, it is believed that B. anthracis spores may survive phagocytosis and may actually use the macrophages that ingest them as a means of transport to lymph nodes. Thus far, the events that occur after spores undergo phagocytosis have remained unclear. To elucidate the fate of spores internalized by macrophages, we have used time-lapse confocal microscopy to follow individual fluorescent spores over time. By use of this method, we have determined that some phagocytized spores survive beyond germination, to become bacilli that then replicate within the macrophages. |
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Authors:
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Gordon Ruthel; Wilson J Ribot; Sina Bavari; Timothy A Hoover |
Publication Detail:
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Type: Journal Article Date: 2004-03-19 |
Journal Detail:
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Title: The Journal of infectious diseases Volume: 189 ISSN: 0022-1899 ISO Abbreviation: J. Infect. Dis. Publication Date: 2004 Apr |
Date Detail:
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Created Date: 2004-03-19 Completed Date: 2004-05-13 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0413675 Medline TA: J Infect Dis Country: United States |
Other Details:
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Languages: eng Pagination: 1313-6 Citation Subset: AIM; IM |
Affiliation:
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Clinical Research Management, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland 21702, USA. gordon.ruthel@amedd.army.mil. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacillus anthracis / growth & development, immunology, physiology* Carbocyanines / chemistry Fluorescent Dyes / chemistry Macrophages / immunology, microbiology* Mice Microscopy, Confocal Organic Chemicals Phagocytosis / physiology Spores, Bacterial |
| Chemical | |
Reg. No./Substance:
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0/3,3'-dihexadecylindocarbocyanine; 0/Carbocyanines; 0/Fluorescent Dyes; 0/Organic Chemicals; 0/SYTOX Green |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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